Procesamiento de la hoja de Kalanchoe gastonis bonnieri y aloe vera, para obtener unabebida nutracética
Fecha
2016
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Universidad de Guayaquil, Facultad de Ingeniería Química
Resumen
Se procesó la hoja de Kalanchoe gastonis bonnieri, familia Crassulaceae para elaborar una bebida nutracéutica. Se recolectó 5 kg de hojas, se sometieron a un proceso de secado a 60°C, con una velocidad de 1.335 kg/m2 y humedad en base seca de 5,47%. Se realizó la extracción con metanol 98% donde se obtuvo un rendimiento de extracto del 7,34 %. El tamizaje fitoquímico en forma cualitativa obtuvo los siguientes resultados: resinas (-), fehling (+), espuma (+), cloruro férrico (+), antocianidinas (-), shinoda (+), Dragendorff (+), Mayer (+). Por los métodos de GC/MS o HPLC se ha aislado la nueva quercetina flavonoide 3-O-α-ramnopiranósido-7-O-β-D-glucopiranosil (1 -> 3) -α -L-ramnopiranósido, así como la ya conocida 6-C-β-D-glucopiranosil 8-C-β-D-glucopyranosylapigenin (vicenin-2). El ensayo de radicales libres por el método del DPPH a 517 nm en el espectrofotómetro Genesys 10 uv da 92.25% con 50 μL con extracto de hoja seca. La bebida objeto del proyecto, resultó de la siguiente mezcla: 88.80% aloe vera, 10% hoja Kalanchoe gastonis bonnieri, 1.20% esteviosidos. Realizamos la determinación final de antioxidantes presentes en el producto, donde nos reportó un 87,48% de inhibición con 200 μL. El estudio de la estabilidad acelerada se lo determinó a 45 °C resultando 12 días equivalentes a 4 meses. El porcentaje de aceptación mediante una encuesta a 30 personas reportando un 70% de aprobación y un 30% de rechazo
Leaf kalanchoe gastonis-bonnieri, family Crassulaceae was processed to produce a nutraceutical beverage. 5 kg of leaves were collected, subjected to a drying process at 60 ° C at a rate of 1335 kg / m2 on dry basis moisture of 5.47%. Methanol extraction where 98% yield of 7.34% extract obtained was performed. The phytochemical screening qualitatively obtained the following results: resins (-), fehling (+), foam (+), ferric chloride (+), anthocyanins (-), shinoda (+), Dragendorff (+), Mayer (+). By the methods of GC / MS or HPLC it has been isolated flavonoid quercetin new 3-O-α-rhamnopyranoside-7-O-β-D-glucopyranosyl (1 -> 3) -α-L-rhamnopyranoside, as well as the already known 6-C-β-D-glucopyranosyl-8-C-β-D glucopyranosylapigenin (vicenin-2). The assay of free radicals by the method of DPPH at 517 nm in the spectrophotometer Genesys 10 uv gives 92.25% with 50 μL dry leaf extract. The drink under the project, resulted from the following mixture: 88.80% aloe vera, 10% leaf kalanchoe gastonis-bonnieri, and 1.20% steviosides. We perform the final determination of antioxidants present in the product, where we reported a 87.48% inhibition with 200 μL. The study of accelerated stability as determined at 45 ° C resulting 12 days equivalent to 4 months. The acceptance rate through a survey of 30 people reporting a 70% approval and 30% rejection.
Leaf kalanchoe gastonis-bonnieri, family Crassulaceae was processed to produce a nutraceutical beverage. 5 kg of leaves were collected, subjected to a drying process at 60 ° C at a rate of 1335 kg / m2 on dry basis moisture of 5.47%. Methanol extraction where 98% yield of 7.34% extract obtained was performed. The phytochemical screening qualitatively obtained the following results: resins (-), fehling (+), foam (+), ferric chloride (+), anthocyanins (-), shinoda (+), Dragendorff (+), Mayer (+). By the methods of GC / MS or HPLC it has been isolated flavonoid quercetin new 3-O-α-rhamnopyranoside-7-O-β-D-glucopyranosyl (1 -> 3) -α-L-rhamnopyranoside, as well as the already known 6-C-β-D-glucopyranosyl-8-C-β-D glucopyranosylapigenin (vicenin-2). The assay of free radicals by the method of DPPH at 517 nm in the spectrophotometer Genesys 10 uv gives 92.25% with 50 μL dry leaf extract. The drink under the project, resulted from the following mixture: 88.80% aloe vera, 10% leaf kalanchoe gastonis-bonnieri, and 1.20% steviosides. We perform the final determination of antioxidants present in the product, where we reported a 87.48% inhibition with 200 μL. The study of accelerated stability as determined at 45 ° C resulting 12 days equivalent to 4 months. The acceptance rate through a survey of 30 people reporting a 70% approval and 30% rejection.
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Kalanchoe gastonis bonnieri, Nutracéutica, Flavonoide, Antioxidante