Evaluación de la actividad antimicrobiana y antioxidante de los extractos obtenidos del escarabajo chino (Ulomoides dermestoides)
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2018
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Universidad de Guayaquil. Facultad de Ciencias Químicas
Resumen
El presente trabajo tiene como objetivo evaluar la actividad antimicrobiana y antioxidante “in vitro” de los extractos obtenidos del Ulomoides dermestoides. Los parámetros de calidad de la droga fueron realizados en el Laboratorio de Productos Naturales de la Facultad de Ciencias Químicas, donde se obtuvieron los siguientes resultados: 54.37% humedad, 1.61% cenizas totales, 2.60% cenizas solubles en agua, 0.50% cenizas insolubles en HCl. De sólidos totales se obtuvieron 11.5 % en hexano, 4.36 % en acetato de etilo, 5.36 % en metanol y 5.46 % Agua.. El tamizaje fitoquímico evidenció la presencia de grasa, triterpenos y esteroides, quinonas, catequinas y resinas. Se obtuvieron extractos con polaridades crecientes como: hexano, acetato de etilo, metanol y agua. Los ensayos de la actividad antimicrobiana se realizaron frente a patógenos de interés en el área de salud, recomendados por el Instituto Nacional de investigación en salud pública (INSPI), esta se realizó empleando el método de difusión Kirby Bauer, para los extractos hexanico y de Acetato de etilo, determinando que el extracto hexanico presenta la mayor capacidad antimicrobiana. Mientras que los extractos Acuoso y Metanólico, se empleó el método de Concentración Mínima Inhibitoria (CMI), donde el extracto acuoso ejerció actividad antimicrobiana frente al Staphylococcus aureus 25923. Se evalúo la actividad antioxidante del extracto alcohólico, mediante tres diferentes métodos, como el DPPH+ donde se obtuvo un porcentaje de actividad antioxidante del 47,21% y una concentración de 24 mg/mL EAG, Para corroborar el valor se determinó el contenido de Fenoles Totales obteniendo 2,4 mg/mL EAG, y de Flavonoides totales expresados en quercetina dando 6,44 mg/mL
The objective of this work is to evaluate the antimicrobial and antioxidant activity "in vitro" of the extracts obtained from Ulomoides dermestoides. The parameters of quality of the drug were made in the Natural Products Laboratory of the Faculty of Chemical Sciences, where they obtained the following results: 54.37% humidity, 1.61% total ash, 2.60% ash soluble in water, 0.50% insoluble ashes in HCl . It is estimated that 11.5% in hexane, 4.36% in ethyl acetate, 5.36% in methanol and 5.46% in water. Phytochemical screening is the presence of fat, triterpenes and steroids, quinones, catechins and resins. Extracts were obtained with increasing polarities such as: hexane, ethyl acetate, methanol and water. The tests of the antimicrobial activity were carried out against pathogens of interest in the health area, recommended by the National Institute of Public Health Research (INSPI), this was done using the Kirby Bauer diffusion method, for the hexane and extract extracts. Ethyl acetate, determining that the hexane extract has the highest antimicrobial capacity. While Aqueous and Methanolic extracts, the method of Minimum Inhibitory Concentration (MIC) was used, where the aqueous extract exerted the antimicrobial activity against Staphylococcus aureus 25923. The antioxidant activity of the alcoholic extract was evaluated, through three different methods, as the DPPH + where a percentage of antioxidant activity of 47.21% and a concentration of 24 mg / mL EAG was obtained. To confirm the value, the content of Total Phenols was determined, obtaining 2.4 mg / mL EAG, and Flavonoids total expressed in quercetin giving 6.44 mg / mL
The objective of this work is to evaluate the antimicrobial and antioxidant activity "in vitro" of the extracts obtained from Ulomoides dermestoides. The parameters of quality of the drug were made in the Natural Products Laboratory of the Faculty of Chemical Sciences, where they obtained the following results: 54.37% humidity, 1.61% total ash, 2.60% ash soluble in water, 0.50% insoluble ashes in HCl . It is estimated that 11.5% in hexane, 4.36% in ethyl acetate, 5.36% in methanol and 5.46% in water. Phytochemical screening is the presence of fat, triterpenes and steroids, quinones, catechins and resins. Extracts were obtained with increasing polarities such as: hexane, ethyl acetate, methanol and water. The tests of the antimicrobial activity were carried out against pathogens of interest in the health area, recommended by the National Institute of Public Health Research (INSPI), this was done using the Kirby Bauer diffusion method, for the hexane and extract extracts. Ethyl acetate, determining that the hexane extract has the highest antimicrobial capacity. While Aqueous and Methanolic extracts, the method of Minimum Inhibitory Concentration (MIC) was used, where the aqueous extract exerted the antimicrobial activity against Staphylococcus aureus 25923. The antioxidant activity of the alcoholic extract was evaluated, through three different methods, as the DPPH + where a percentage of antioxidant activity of 47.21% and a concentration of 24 mg / mL EAG was obtained. To confirm the value, the content of Total Phenols was determined, obtaining 2.4 mg / mL EAG, and Flavonoids total expressed in quercetin giving 6.44 mg / mL
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EXTRACTO, POLARIDAD, ANTIMICROBIANO, ANTIOXIDANTES