Análisis por el método SCD del nivel de fragmentación de ADN espermático de la muestra poblacional de hombres en Guayaquil.
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2018
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Facultad de Ciencias Naturales, Universidad de Guayaquil
Resumen
Una de las causas de infertilidad masculina es la fragmentación del ADN espermático. En este trabajo se evaluaron pacientes por espermatograma y Dispersión de la Cromatina Espermática (SCD). Para esto, se sondearon con pruebas de rutina a 75 individuos, de los cuales 43 (57%) fueron considerados para el estudio. Se clasificaron en cuatro grupos (G) de estudio, considerando las actividades laborales, siendo las áreas cerradas para G1, al aire libre G2, fumadores G3 y, control G4. En la correlación total de los Factores de Incidencia Intergénica (FII), la más alta fue entre el criterio de la edad con medicamentos (p=0.9) en G3. En cuanto al análisis de afinidad de todas las morfoestructuras espermáticas (MEE); el 76% dio una correlación de p=+1,-1. De acuerdo a la asignación estimada por los componentes principales; G-1 demuestra una asociación positiva entre defecto de cabeza y cuello. Por otro lado, el Índice de Fragmentación (IDF) con mayor incidencia negativa se encontró en G-3 con el 29%. Finalmente la MEE y, la fragmentación del ADN presenta diferencia no significativa, además se logró observar que la infertilidad provocada por la fragmentación del ADN fue del 20%.
One of the causes of male infertility is spermatic DNA fragmentation. In this work, patients were evaluated by spermatograms and Sperm Chromatin Dispersion (SCD). This is plumbed with routine testing to 75 individuals, of which 43(57%) were considered for the study. They were classified in to four study groups (G), considering work activities, being closed areasG1, open airsG2, smokingG3 and control G4. In total correlation of the factors of intergenic incidence (FII), the highest was from the criterion of age with medicines (p=0.9) in G3. Analysis of affinity of all sperm morfoestructuras (MEE); 76% gave a correlation of p= +1, -1. According to the allocation estimated by main components; G1 shows a positive association between defects in the head and neck. On the other hand, the fragmentation index (IDF) with increased negative incidence was found in G3 with 29%. Finally, the MEE and the fragmentation of the DNA present no significant difference, also managed to observe that the infertility caused by the fragmentation of the DNA was 20%
One of the causes of male infertility is spermatic DNA fragmentation. In this work, patients were evaluated by spermatograms and Sperm Chromatin Dispersion (SCD). This is plumbed with routine testing to 75 individuals, of which 43(57%) were considered for the study. They were classified in to four study groups (G), considering work activities, being closed areasG1, open airsG2, smokingG3 and control G4. In total correlation of the factors of intergenic incidence (FII), the highest was from the criterion of age with medicines (p=0.9) in G3. Analysis of affinity of all sperm morfoestructuras (MEE); 76% gave a correlation of p= +1, -1. According to the allocation estimated by main components; G1 shows a positive association between defects in the head and neck. On the other hand, the fragmentation index (IDF) with increased negative incidence was found in G3 with 29%. Finally, the MEE and the fragmentation of the DNA present no significant difference, also managed to observe that the infertility caused by the fragmentation of the DNA was 20%
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CONTAMINACIÓN AMBIENTAL, DEGRADACIÓN, ESPERMATOZOIDE, INFERTILIDAD