Evaluación in vitro de la actividad antibacteriana y antioxidante de los extractos de Malva sylvestris y Malva pseudolavatera
Fecha
2019
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Universidad de Guayaquil. Facultad de Ciencias Químicas
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En el presente trabajo de investigación se realizó la evaluación de la actividad antibacteriana de extractos acuosos e hidroalcohólicos de las hojas de Malva sylvestris y Malva pseudolavatera, contra tres microorganismos (Escherichia coli, Staphylococcus aureus, Klebsiella pneumoniae) y la evaluación antioxidante de los extractos hidroalcohólicos, con la finalidad de brindar una alternativa en el tratamiento de enfermedades infecciosas y crónico-degenerativas. La actividad antibacteriana se realizó por ensayos de difusión en discos utilizando Cefotaxima como antibiótico de referencia; la actividad antioxidante se llevó a cabo mediante tres métodos in vitro, el método de FRAP (capacidad ferroreductora), 2,2-difenil-
1-picrilhidracilo (DPPH) y ácido 2,2’-azino-bis (3-etilbenzotiazolina)-6-sulfónico (ABTS•+). Los resultados obtenidos indicaron que los extractos analizados de ambas especies de Malva presentaron una actividad antibacteriana moderada en determinadas concentraciones frente a los microorganismos E. coli, S. aureus y K. pneumoniae; y teniendo en consideración los resultados de la actividad antioxidante en los tres métodos in vitro ensayados se pudo constatar que a medida que aumentaba la concentración de los extractos, aumentaba el poder reductor (ensayo FRAP) y la actividad antirradicalaria (ensayos DPPH y ABTS•+) de los mismos, manifestándose una elevada actividad antioxidante. Como consecuencia de esto, podemos concluir que los extractos de las hojas de Malva sylvestris y Malva pseudolavatera producen halos de inhibición en las cepas de los microorganismos evaluados (E. coli, S. aureus, K. pneumoniae), además de presentar inhibición de los radicales empleados (DPPH, ABTS•+) y capacidad ferro-reductora (FRAP).
In the present research work has done the evaluation of the antibacterial activity of aqueous and hydroalcoholic extracts of the leaves of Malva sylvestris and Malva pseudolavatera, against three microorganisms (Escherichia coli, Staphylococcus aureus, Klebsiella pneumoniae) and antioxidant evaluation of hydroalcoholic extracts, with the goal to provide an alternative in the treatment of infectious and chronic degenerative diseases. The antibacterial activity was performed by disk diffusion assays using Cefotaxime as a reference antibiotic; The antioxidant activity was carried out by three in vitro methods, the method of FRAP (ferro-reducing capacity), 2,2-diphenyl-1-picrylhydracil (DPPH) and 2,2'- azino-bis (3-ethylbenzothiazoline)-6-sulfonic (ABTS•+). The results obtained indicated that the analyzed extracts of both Malva species showed a moderate antibacterial activity in certain concentrations against the microorganisms E. coli, S. aureus and K. pneumoniae; and taking into account the results of the antioxidant activity in the three in vitro methods tested, it was I observed that as the concentration of the extracts increased, the reducing power (FRAP test) and the anti-radicals activity (DPPH and ABTS•+ tests) increased as well, manifesting a high antioxidant activity. As a consequence of this, we can conclude that Malva sylvestris and Malva pseudolavatera leaf extracts produce inhibition halos in the strains of the microorganisms evaluated (E. coli, S. aureus, K. pneumoniae), in addition to presenting inhibition of the radicals used (DPPH, ABTS•+) and ferro- reducing capacity (FRAP).
In the present research work has done the evaluation of the antibacterial activity of aqueous and hydroalcoholic extracts of the leaves of Malva sylvestris and Malva pseudolavatera, against three microorganisms (Escherichia coli, Staphylococcus aureus, Klebsiella pneumoniae) and antioxidant evaluation of hydroalcoholic extracts, with the goal to provide an alternative in the treatment of infectious and chronic degenerative diseases. The antibacterial activity was performed by disk diffusion assays using Cefotaxime as a reference antibiotic; The antioxidant activity was carried out by three in vitro methods, the method of FRAP (ferro-reducing capacity), 2,2-diphenyl-1-picrylhydracil (DPPH) and 2,2'- azino-bis (3-ethylbenzothiazoline)-6-sulfonic (ABTS•+). The results obtained indicated that the analyzed extracts of both Malva species showed a moderate antibacterial activity in certain concentrations against the microorganisms E. coli, S. aureus and K. pneumoniae; and taking into account the results of the antioxidant activity in the three in vitro methods tested, it was I observed that as the concentration of the extracts increased, the reducing power (FRAP test) and the anti-radicals activity (DPPH and ABTS•+ tests) increased as well, manifesting a high antioxidant activity. As a consequence of this, we can conclude that Malva sylvestris and Malva pseudolavatera leaf extracts produce inhibition halos in the strains of the microorganisms evaluated (E. coli, S. aureus, K. pneumoniae), in addition to presenting inhibition of the radicals used (DPPH, ABTS•+) and ferro- reducing capacity (FRAP).
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ANTIBACTERIANO, ANTIOXIDANTES, MALVA SYLVESTRIS, MALVA PSEUDOLAVATERA, RADICALES LIBRES