Evaluación de la concentración de polifenoles totales y actividad antioxidante de las hojas de churco Arthrostemma ciliatum Pav. ex D. Don.
Fecha
2020
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Universidad de Guayaquil. Facultad de Ciencias Químicas
Resumen
La Arthrostemma ciliatum Pav. ex D. Don es una planta que crece en las faldas de la Cordillera de los Andes ecuatorianos, la población consume sus tallos agrios, descartando las hojas, para el tratamiento de afecciones en la garganta, infecciones urinarias, disentería o para calmar la sed. Los radicales libres son átomos muy reactivos que no poseen receptores específicos, tienen una capacidad de agresión indiscriminada sobre células y tejidos vivientes, y están vinculados con las ENT. La composición química de las hojas de la Arthrostemma ciliatum Pav. ex D. Don no ha sido estudiada, por lo cual se realizó un estudio de los metabolitos secundarios mediante un Tamizaje Fitoquímico Preliminar que presentó en el extracto acuoso Triterpenos y/o esteroides, Flavonoides y en extracto etanólico, Alcaloides, Resinas y Taninos Pirocatecólicos. En parámetros fisicoquímicos presentó Cenizas Totales 2.81% y Humedad 77.1%. La concentración de Polifenoles Totales se determinó por el método de Folin-Ciocalteu presentando en extracto acuoso 0.05% y en extracto etanólico 0.11%, la Actividad Antioxidante se evaluó por el método de DPPH presentando en el extracto acuoso 2276.5 mg/g y en el extracto etanólico 1618.5 mg/g.
The Arthrostemma ciliatum Pav. ex D. Don, is a plant that grows on the slopes of the Ecuadorian Andes, the population consumes its sour stalks, discarding the leaves, for the treatment of throat conditions, urinary infections, dysentery or to quench thirst. Free radicals are very reactive atoms that do not have specific receptors, have a capacity for indiscriminate aggression on living cells and tissues, and are linked to NCDs. The chemical composition of the leaves of the Arthrostemma ciliatum Pav. ex D. Don has not been studied, so a study of the secondary metabolites was carried out by means of a Preliminary Phytochemical Screening that presented in the aqueous extract Triterpenes and/or steroids, Flavonoids and in ethanolic extract, Alkaloids, Resins and Pyrocatecholic Tannins. In physicochemical parameters, it presented Total Ashes 2.81% and Humidity 77.1%. The concentration of Total Polyphenols was determined by the Folin-Ciocalteu method presenting 0.05% aqueous extract and 0.11% ethanol extract, the Antioxidant Activity was evaluated by the DPPH method presenting 2276.5 mg/g aqueous extract and ethanolic extract 1618.5 mg/g.
The Arthrostemma ciliatum Pav. ex D. Don, is a plant that grows on the slopes of the Ecuadorian Andes, the population consumes its sour stalks, discarding the leaves, for the treatment of throat conditions, urinary infections, dysentery or to quench thirst. Free radicals are very reactive atoms that do not have specific receptors, have a capacity for indiscriminate aggression on living cells and tissues, and are linked to NCDs. The chemical composition of the leaves of the Arthrostemma ciliatum Pav. ex D. Don has not been studied, so a study of the secondary metabolites was carried out by means of a Preliminary Phytochemical Screening that presented in the aqueous extract Triterpenes and/or steroids, Flavonoids and in ethanolic extract, Alkaloids, Resins and Pyrocatecholic Tannins. In physicochemical parameters, it presented Total Ashes 2.81% and Humidity 77.1%. The concentration of Total Polyphenols was determined by the Folin-Ciocalteu method presenting 0.05% aqueous extract and 0.11% ethanol extract, the Antioxidant Activity was evaluated by the DPPH method presenting 2276.5 mg/g aqueous extract and ethanolic extract 1618.5 mg/g.
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ARTHROSTEMMA CILIATUM PAV. EX D. DON, POLIFENOLES TOTALES, ACTIVIDAD ANTIOXIDANTE, TAMIZAJE FITOQUÍMICO PRELIMINAR