Detección de sars-cov-2, Escherichia coli y coliformes fecales en el estero Data de Posorja, provincia Guayas-Ecuador
Fecha
2021-03
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Facultad de Ciencias Naturales. Universidad de Guayaquil
Resumen
La finalidad de esta investigación fue determinar la presencia de SARS-CoV-2, Escherichia coli y coliformes fecales en el estero Data de Posorja, mediante la recolección de muestras de coliformes fecales en 4 sitios por triplicado y las muestras de SARS-CoV-2 obtenidas de forma puntual en los 4 sitios y en una salida de aguas residuales y la aplicación de las metodologías de la prueba de Número Más Probable y el análisis RT-qPCR, respectivamente. Se determinaron concentraciones de coliformes fecales de 10,1 a 192 NMP/100 mL, con concentraciones máximas de 460 NMP/100 mL, Escherichia coli de 6,5 a 50,7 NMP/100 mL y no se detectó cargas virales de SARS-CoV-2. Se tomaron parámetros fisicoquímicos de pH, temperatura en agua, salinidad, oxígeno disuelto, conductividad eléctrica y total de sólidos disueltos in situ, mostrando correlación entre dichos parámetros. Las máximas obtenidas demostraron contaminación fecal, además, se presentaron valores bajos de oxígeno disuelto y niveles altos de salinidad indicando que el agua se encuentra contaminada y no es apta para fines recreativos mediante contacto primario.
The purpose of this research was to determine the presence of SARS-CoV-2, Escherichia coli and fecal coliforms in the Data of Posorja estuary. Samples of fecal coliforms were collected in 4 sites by triplicate, SARS-CoV-2 samples were obtained in a timely manner at the 4 sites and one at the wastewater outlet by using the NMP test and the RT-qPCR analysis, respectively. Fecal coliform concentrations were determined from 10.1 to 192 MPN/100 mL, with a maximum of 460 MPN/100 mL, Escherichia coli from 6.5 to 50.7 MPN/100 mL; SARS- CoV-2 was not detected. Physicochemical parameters on pH, temperature in water, salinity, dissolved oxygen, electrical conductivity, and total dissolved solids were taken in situ showing correlation. The maximums showed fecal contamination, in addition, there were low values of dissolved oxygen and high levels of salinity indicating that the water is contaminated and is not suitable for recreational purposes.
The purpose of this research was to determine the presence of SARS-CoV-2, Escherichia coli and fecal coliforms in the Data of Posorja estuary. Samples of fecal coliforms were collected in 4 sites by triplicate, SARS-CoV-2 samples were obtained in a timely manner at the 4 sites and one at the wastewater outlet by using the NMP test and the RT-qPCR analysis, respectively. Fecal coliform concentrations were determined from 10.1 to 192 MPN/100 mL, with a maximum of 460 MPN/100 mL, Escherichia coli from 6.5 to 50.7 MPN/100 mL; SARS- CoV-2 was not detected. Physicochemical parameters on pH, temperature in water, salinity, dissolved oxygen, electrical conductivity, and total dissolved solids were taken in situ showing correlation. The maximums showed fecal contamination, in addition, there were low values of dissolved oxygen and high levels of salinity indicating that the water is contaminated and is not suitable for recreational purposes.
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COLIFORMES FECALES, ESCHERICHIA coli, ARS-CoV-2.