Estudio microbiológico de Staphylococcus aureus en transportes urbanos que circulan en la ciudad de Guayaquil.
Fecha
2022
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Universidad de Guayaquil. Facultad de Ciencias Químicas
Resumen
El objetivo del presente estudio es analizar la carga microbiana que se encuentra en los buses urbanos en la ciudad de Guayaquil en relación a las superficies de contactos más usadas por los usuarios y los efectos que podrían generar en la salud. La presente investigación es de tipo cuantitativo, con diseño no experimental con enfoque transversal, realizado con una muestra de 40 buses urbanos en el sector norte y centro donde existe más afluencia de personas. Se realizó hisopados por medio del método IMES para recolección de 40 muestras, posterior se realizó siembra en medio agar baird Parker en los laboratorios UBA-LAB. En 10 de 40 muestras analizadas se encontró que las UFC/cm2 fueron mayor al límite cuantificación de 10
UFC/cm2 para Staphylococcus aureus, por lo que se considera que el riesgo
de contaminación es del 25% para todos los usuarios que frecuenta los buses urbanos de la ciudad de Guayaquil.
The objective of this study is to analyze the microbial load found in urban buses in the city of Guayaquil in relation to the contact surfaces most used by users and the effects they could generate on health. This is a quantitative research, with a non-experimental design with a cross-sectional approach, carried out with a sample of 40 urban buses in the north and center sectors where there is more affluence of people. Swabs were taken using the IMES method for the collection of 40 samples, followed by sowing in Baird Parker agar medium at the UBA-LAB laboratories. In 10 of the 40 samples analyzed, it was found that the CFU/cm2 were higher than the quantification limit of 10 CFU/cm2 for Staphylococcus aureus, so it is considered that the risk of contamination is 25% for all users who frequent urban buses in the city of Guayaquil.
The objective of this study is to analyze the microbial load found in urban buses in the city of Guayaquil in relation to the contact surfaces most used by users and the effects they could generate on health. This is a quantitative research, with a non-experimental design with a cross-sectional approach, carried out with a sample of 40 urban buses in the north and center sectors where there is more affluence of people. Swabs were taken using the IMES method for the collection of 40 samples, followed by sowing in Baird Parker agar medium at the UBA-LAB laboratories. In 10 of the 40 samples analyzed, it was found that the CFU/cm2 were higher than the quantification limit of 10 CFU/cm2 for Staphylococcus aureus, so it is considered that the risk of contamination is 25% for all users who frequent urban buses in the city of Guayaquil.
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SUPERFICIES DE CONTACTO, BUSES URBANOS, UNIDADES FORMADORAS DE COLONIA, NIVEL DE CONTAMINACIÓN Y MICROORGANISMO