Cepas de candida albicans, aisladas en pacientes con diabetes mellitus y su resistencia a los antifúngicos. Hospital del Día IESS
Archivos
Fecha
2014
Autores
Título de la revista
ISSN de la revista
Título del volumen
Editor
Universidad de Guayaquil. Facultad de Ciencias Médicas. Escuela de Graduados
Resumen
En la presente investigación se determinó y aisló las cepas de Candida albicans de muestras a pacientes diabéticos no controlados, con posible recidiva de la infección y se comprobó cualitativamente la inhibición parcial o total a los antifúngicos a través de la prueba de sensibilidad a los antimicóticos administrados. Con una muestra de 170 pacientes diabéticos, seleccionados con criterios de exclusión e inclusión que permitieron obtener los casos más relevantes para el estudio y con métodos estandarizados de recolección, recuperación e identificación, se halló “in vitro” el germen Candida albicans y al mismos tiempo se la confrontó con antimicóticos para medir la sensibilidad micológica. El número de cepas confirmadas para Candida albicans sumaron 132 con una relación porcentual al 78% en relación al total, comparando que por cada 10 personas diabéticas con infecciones micóticas en el estudio, 8 eran causadas efectivamente por la especie albicans y las otras 2, no concordaron con el diagnóstico inicial. A estas mismas cepas se las analizó por el método comercial colorimétrico Integral System Yeasts Plus para comprobar la respectiva sensibilidad revelando que el ketoconazol, el itraconazol y el clotrimazol obtuvieron valores semejantes por debajo del 50% de la sensibilidad óptima, el fluconazol reveló un aumento significativo en los indicadores de resistencia antimicótica del 23% y todos juntos dejaron entrever una progresiva problemática con desafíos a los profesionales de la salud.
In the present investigation identified and isolated strains of Candida albicans samples to uncontrolled diabetic patients with possible worsening of the infection and the partial or total inhibition was qualitatively determined to antifungal agents by susceptibility testing to antifungal administered. With a sample of 170 diabetic patients, selected with inclusion and exclusion criteria, we have obtained the most relevant cases for study and standardized collection, recovery and identification methods, "in vitro" it was found on Candida albicans germ and the same time he confronted her with antifungal mycological measuring sensitivity. The number of strains confirmed to Candida albicans totaled 132 with a percentage ratio of 78% relative to the total, compared to per 10 people with diabetes with fungal. Infections in the study 8 were actually caused by albicans species and the other two, not they agreed with the initial diagnosis. These same strains were screen by the colorimetric commercial method Integral System Yeasts Plus to check the respective sensitivity revealing that ketoconazole, itraconazole and clotrimazole obtained similar values below 50% of the optimal sensitivity, fluconazole revealed a significant increase indicator of antifungal resistance of 23% and together hinted a progressive problem with challenges to health professionals
In the present investigation identified and isolated strains of Candida albicans samples to uncontrolled diabetic patients with possible worsening of the infection and the partial or total inhibition was qualitatively determined to antifungal agents by susceptibility testing to antifungal administered. With a sample of 170 diabetic patients, selected with inclusion and exclusion criteria, we have obtained the most relevant cases for study and standardized collection, recovery and identification methods, "in vitro" it was found on Candida albicans germ and the same time he confronted her with antifungal mycological measuring sensitivity. The number of strains confirmed to Candida albicans totaled 132 with a percentage ratio of 78% relative to the total, compared to per 10 people with diabetes with fungal. Infections in the study 8 were actually caused by albicans species and the other two, not they agreed with the initial diagnosis. These same strains were screen by the colorimetric commercial method Integral System Yeasts Plus to check the respective sensitivity revealing that ketoconazole, itraconazole and clotrimazole obtained similar values below 50% of the optimal sensitivity, fluconazole revealed a significant increase indicator of antifungal resistance of 23% and together hinted a progressive problem with challenges to health professionals
Descripción
PDF
Palabras clave
Candida albicans, Diabetes mellitus, Hospital del Día Dr. Efrén Jurado López (IESS), Cantón Guayaquil, Ecuador, Anticuerpos antifúngicos