Aclimatización de plantulas de Vainilla Tahitensis (Orchidaceae) propagadas ‘‘in vitro’’
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2022
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Universidad de Guayaquil : Facultad de Ciencias Agrarias
Resumen
En la siguiente investigación se busca observar los objetivos generales basados en desarrollar un protocolo para aclimatación de plántulas de Vainilla tahitensis (Orchidaceae) propagadas ‘‘in vitro’’ a partir de ápices apicales y axilares. El presente ensayo fue realizado en AGROVITROPARIS ubicado en Guayaquil provincia del Guayas, donde se empleó un diseño de bloques completamente al azar. Se utilizó seis tratamientos de diferentes sustratos. estudiando el número de hojas, número de entrenudos, diámetro de la hoja. Se adquirieron las siguientes conclusiones, con la aplicación de perlita, humus de lombriz, carbón vegetal, turba, vermiculita y fibra de coco, las mejores plantas que se obtuvieron fueron en el tratamiento tres con un número de hoja de 49 y un número de entrenudo 2,8, mientras que el tratamiento cuatro fue el más económico a comparación del resto con USD 20,00, a diferencia del tratamiento seis el cual si reflejo más costo con USD 65,50.
The following research work seeks to achieve the general aims based on the development of an acclimatization ptotocol of Vanilla tahitensis (Orchidaceae) seedlings propagated "in vitro" from apical and axillary apices. The current test was conducted at AGROVITROPARIS laboratory, which is located in Guayaquil city, Guayas province. Therefore, a completely random block design was applied with six treatments of different substrates as well, so as to study the number of leaves, number of internodes, leaf diameter. The following conclusions were drawn: with the application of perlite, earthworm humus, charcoal, peat, vermiculite and coconut fiber, the best plants that were obtained were in treatment three with a leaf number of 49 and an internode number 2.8, while treatment four was the most economical compared to the rest with USD 20.00, unlike treatment six which was more expensive with USD 65.50.
The following research work seeks to achieve the general aims based on the development of an acclimatization ptotocol of Vanilla tahitensis (Orchidaceae) seedlings propagated "in vitro" from apical and axillary apices. The current test was conducted at AGROVITROPARIS laboratory, which is located in Guayaquil city, Guayas province. Therefore, a completely random block design was applied with six treatments of different substrates as well, so as to study the number of leaves, number of internodes, leaf diameter. The following conclusions were drawn: with the application of perlite, earthworm humus, charcoal, peat, vermiculite and coconut fiber, the best plants that were obtained were in treatment three with a leaf number of 49 and an internode number 2.8, while treatment four was the most economical compared to the rest with USD 20.00, unlike treatment six which was more expensive with USD 65.50.
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Palabras clave
Sustratos, Aclimatización, Aplicación
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APA