Cultivo in vitro de níspero Eriobotryca japónica L. con la interacción de aloe vera y glutamina
Fecha
2018-04
Autores
Título de la revista
ISSN de la revista
Título del volumen
Editor
Facultad de Ciencias Agrarias Universidad de Guayaquil
Resumen
El trabajo de investigación se realizó en el laboratorio de cultivos de
tejidos in vitro AGROVITROPARIS, ubicado en la Av. Piedrahita y Jaime
Roldós, Mz, cantón Daule, Provincia del Guayas; los objetivos fueron: 1)
Encontrar la mejor dosis de glutamina y Aloe vera para la obtención de
brotes in vitro de níspero. 2) Desarrollar un protocolo para la
micropropagación de níspero; para la comparación de las medias se utilizó la
prueba de Duncan al 5% de probabilidad. Las variables evaluadas: longitud
de brotes (cm) y número de brotes presentaron valores estadísticamente
diferentes entre cada uno de los tratamientos estudiados. Se concluyó que:
1) dentro de la investigación las variables evaluadas longitud de brotes (cm)
y número de brotes, presentaron valores estadísticamente diferentes entre
cada uno de los tratamientos; 2) para la variable longitud de brotes (cm) la
mejor dosis la presenta el tratamiento tres con la aplicación de 300 mg/ l -
1 e
Glutamina y 30 mg/ l -
1 e
Aloe vera, mientras que en la variable número de
brotes el mismo tratamiento tres presenta la mejor dosis de aplicación con
300 mg/ l -
1 e
Glutamina y 30 mg/ l -
1 e
Aloe vera; y, 3) el uso de la glutamina y
el Aloe han resultado ser muy eficaces para la obtención explantes in vitro,
convirtiéndose en una herramienta biotecnológica efectiva para la
propagación de especies frutales.
The present research work was carried out in the laboratory of in vitro tissue cultures AGROVITROPARIS, located at Av. Piedrahita and Jaime Roldós, Mz, Canton Daule, Guayas province; The objectives were: 1) Find the best dose of glutamine and Aloe vera for obtaining in vitro medlar buds. 2) Develop a protocol for medlar micropropagation; The Duncan test at 5% probability was applied to compare the means. The variables evaluated were: length of shoots (cm) and number of shoots with statistically different values between each of the treatments under study. It was concluded that: 1) within the research, the variables shoot length (cm) and number of outbreaks presented different statistical values between each of the treatments; 2) for the variable length of shoots (cm), the best dose was presented by treatment three with the application of 300 mg / l -1 e Glutamine and 30 mg / l -1 and Aloe vera, while the variable number of outbreaks presented the best dose of application with 300 mg / l -1 of Glutamine and 30 mg / l -1 of Aloe vera; and, 3) the use of glutamine and Aloe have proved to be very effective for obtaining explants in vitro, becoming an effective biotechnological tool for the propagation of fruit species.
The present research work was carried out in the laboratory of in vitro tissue cultures AGROVITROPARIS, located at Av. Piedrahita and Jaime Roldós, Mz, Canton Daule, Guayas province; The objectives were: 1) Find the best dose of glutamine and Aloe vera for obtaining in vitro medlar buds. 2) Develop a protocol for medlar micropropagation; The Duncan test at 5% probability was applied to compare the means. The variables evaluated were: length of shoots (cm) and number of shoots with statistically different values between each of the treatments under study. It was concluded that: 1) within the research, the variables shoot length (cm) and number of outbreaks presented different statistical values between each of the treatments; 2) for the variable length of shoots (cm), the best dose was presented by treatment three with the application of 300 mg / l -1 e Glutamine and 30 mg / l -1 and Aloe vera, while the variable number of outbreaks presented the best dose of application with 300 mg / l -1 of Glutamine and 30 mg / l -1 of Aloe vera; and, 3) the use of glutamine and Aloe have proved to be very effective for obtaining explants in vitro, becoming an effective biotechnological tool for the propagation of fruit species.
Descripción
Palabras clave
TEJIDOS Y VITRO, PROPAGACIÓN IN VITRO