Listeria monocytogenes en canales de vacuno, control microbiológico en la Empresa Embutidos La Italiana, agosto - septiembre del 2015.
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2017
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Universidad de Guayaquil. Facultad de Ciencias Médicas. Escuela de Graduados
Resumen
Introducción. La bacteria Listeria monocytogenes, es una bacteria gram positiva,
no esporulada, aerobia anaerobia facultativa, puede ingresar a las plantas de
procesamiento a través de la materia prima cárnica, y así llegar a la población vulnerable.
Objetivo general: determinar Listeria monocytogenes en canales de vacuno,
control microbioloógico en la empresa Embutidos La Italiana, durante el periodo
agosto – septiembre del año 2015. Objetivos específicos Aislar e identificar la
Listeria monocytogenes en las muestras de carne fresca en las canales de carne de
vacuno, durante la primera fase de recepción de la materia prima cárnica,
mediante el método de aislamiento/enriquecimiento. Aislar e identificar la Listeria
monocytogenes en las muestras de carne fresca en las canales de carne de vacuno,
al final de la segunda fase de recepción de materia prima cárnica, mediante el
método de aislamiento/enriquecimiento. Determinar la distribución de la
contaminación bacteriana en las canales de vacuno, en el área de recepción de
materia prima cárnica de la empresa. Diseñar el protocolo de diagnóstico para
Listeria monocytogenes. La metodología utilizada es cuantitativa descriptiva, que
incluye el enriquecimiento, inmuno-precipitación, e identificación bioquímica.
Los resultados encontrados fueron que la bacteria Listeria monocytogenes tiene
una presencia del 2.5% sobre los canales de vacuno, también se ha demostrado
que el proceso de limpieza tiene un efecto drástico sobre la flora inicial y queda
reducida en dos números logarítmicos. El protocolo para el diagnóstico e
identificación de la bacteria es el adecuado.
Introduction. The bacterium Listeria monocytogenes, is a Gram-positive, non-spore bacteria, aerobic facultative, anaerobic can enter to the processing plants through the raw meat, and thus reach the vulnerable population. General objective: determine Listeria monocytogenes in channels of beef, microbiological control in the Italian sausage company, during the period August - September of the year 2015. Specific objectives to isolate and identify the Listeria monocytogenes in samples of fresh meat in the carcasses of beef during the first phase of the meat raw, using the method of isolation/enrichment. Isolate and identify the Listeria monocytogenes in samples of fresh meat in the carcasses of beef, at the end of the second phase of raw meat, using the method of isolation/enrichment. Determine the distribution of bacterial contamination in carcases of cattle, in the area of reception of raw meat of the company. Design the diagnostic Protocol for Listeria monocytogenes. The methodology is quantitative descriptive, which includes enrichment, immuno-precipitation, and biochemical identification. The found results were that the bacterium Listeria monocytogenes has a presence of 2.5% over beef channels, has also been shown that the cleaning process has a drastic effect on the initial flora and is reduced in two logarithmic numbers. Protocol for diagnosis and identification of the bacterium is suitable.
Introduction. The bacterium Listeria monocytogenes, is a Gram-positive, non-spore bacteria, aerobic facultative, anaerobic can enter to the processing plants through the raw meat, and thus reach the vulnerable population. General objective: determine Listeria monocytogenes in channels of beef, microbiological control in the Italian sausage company, during the period August - September of the year 2015. Specific objectives to isolate and identify the Listeria monocytogenes in samples of fresh meat in the carcasses of beef during the first phase of the meat raw, using the method of isolation/enrichment. Isolate and identify the Listeria monocytogenes in samples of fresh meat in the carcasses of beef, at the end of the second phase of raw meat, using the method of isolation/enrichment. Determine the distribution of bacterial contamination in carcases of cattle, in the area of reception of raw meat of the company. Design the diagnostic Protocol for Listeria monocytogenes. The methodology is quantitative descriptive, which includes enrichment, immuno-precipitation, and biochemical identification. The found results were that the bacterium Listeria monocytogenes has a presence of 2.5% over beef channels, has also been shown that the cleaning process has a drastic effect on the initial flora and is reduced in two logarithmic numbers. Protocol for diagnosis and identification of the bacterium is suitable.
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Enfermedades de los bovinos, Listeria monocytogenes, Bacterias, Empresa Embutidos La Italiana, Cantón Cuenca, Ecuador, Control de microorganismos