Análisis bibliográfico de la determinación de campylobacter en pollos por medio de la técnica PCR en países de Sudamérica
Fecha
2021
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Universidad de Guayaquil. Facultad de Ciencias Químicas
Resumen
Actualmente, el género Campylobacter comprende 17 especies y seis subespecies, de las cuales las detectadas con más frecuencia en enfermedades humanas son C. jejuni (subespecie jejuni) y C. coli. Campylobacter es una de las cuatro principales causas mundiales de enfermedad diarreica y está considerada como la causa bacteriana más frecuente de gastroenteritis en el mundo. Las infecciones por Campylobacter suelen ser leves, pero pueden ser mortales en niños muy pequeños, personas de edad e individuos inmunodeprimidos. En el presente estudio bibliográfico se da a conocer sobre las enfermedades y síntomas causadas por el Campylobacter spp. en los seres humanos por consumo de carnes de pollo, a su vez, se comparan las diferentes técnicas PRC usadas en los países tales como Chile, Perú, Colombia y Ecuador, en el que se concluye que en las muestras de pollo existen altos valores de C. Jejuni, C. coli y C. spp. siendo la técnica más eficiente la PCR multiplex debido a que mediante esta técnica se determinan múltiples fragmentos de ADN en una sola
reacción y en la misma muestra, por lo tanto, ahorra tiempo y es más eficaz.
Currently, the genus Campylobacter includes 17 species and six subspecies, of which the most frequently detected in humans, include C. jejuni (subspecies jejuni) and C. coli. Campylobacter is one of the four main global causes of diarrheal disease and is considered the most common bacterial cause of gastroenteritis in the world. Campylobacter infections are usually mild, but can be fatal in very young children, the elderly, and immunosuppressed individuals. In the present bibliographic study to learn about the diseases and symptoms caused by Campylobacter spp. in human beings by consumption of chicken meat, in turn, they compare the different PRC techniques used in countries such as Chile, Peru, Colombia and Ecuador, in which it is concluded that in chicken samples there are high values of C Jejuni, C. coli and C. spp. Multiplex PCR being the most efficient technique because this technique determines multiple DNA fragments in a single reaction and in the same sample, therefore, it saves time and is more efficient.
Currently, the genus Campylobacter includes 17 species and six subspecies, of which the most frequently detected in humans, include C. jejuni (subspecies jejuni) and C. coli. Campylobacter is one of the four main global causes of diarrheal disease and is considered the most common bacterial cause of gastroenteritis in the world. Campylobacter infections are usually mild, but can be fatal in very young children, the elderly, and immunosuppressed individuals. In the present bibliographic study to learn about the diseases and symptoms caused by Campylobacter spp. in human beings by consumption of chicken meat, in turn, they compare the different PRC techniques used in countries such as Chile, Peru, Colombia and Ecuador, in which it is concluded that in chicken samples there are high values of C Jejuni, C. coli and C. spp. Multiplex PCR being the most efficient technique because this technique determines multiple DNA fragments in a single reaction and in the same sample, therefore, it saves time and is more efficient.
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CAMPYLOBACTER SPP, PCR (CADENA DE POLIMERASA)