Diversidad de transcriptos asociados en la respuesta inmunitaria del camarón Litopenaeus vannamei.
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2015
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El presente trabajo se lo realizó con las muestras obtenidas de un Laboratorio de maduración de la zona de Mar Bravo localizada en la zona costera de la Provincia de Santa Elena, el objetivo de este proyecto fue el estudio del estado inmunitario del camarón L. vannamei, el cual mediante un reconocimiento oportuno y la utilización de la bioinformática, se presentan como una nueva metodología para mejorar las condiciones del cultivo de camarón.
En este estudio se realizó un banco de ADNc, con el propósito de revisar los transcriptos relacionados con la función del sistema inmune del camarón. L. vannamei. Para la obtención de los transcriptos se siguió la metodología ARNseq Análisis de Secuenciación de Próxima Generación (NGS) con sus siglas en inglés, y las lecturas fueron sometidas al análisis TRINITY para el ensamblaje de Novo.
Dentro de los transcriptos que se encontraron en el banco shotgun 440 fueron seleccionados de tipo inmunitario. De esta selección se obtuvieron 336 transcriptos asociados al sistema inmune con valores significativos de probabilidad, a partir de este grupo de genes se procedió a realizar su clasificación de acuerdo a su función inmune.
Así se pudieron obtener transcriptos asociados a Endocitosis, Función de defensa a Virus, Función de defensa a Bacterias, Función de defensa a hongos, Función humoral, Cascadas de señalización de respuesta inmune y otras funciones inmunitarias.
This thesis was conducted with the samples obtained from a maturation laboratory that is located in the coastal area of Mar Bravo in the Province of Santa Elena, the aim of this project was to study the immune status of the shrimp L. vannamei, which an early recognition and use of bioinformatics, they are presented as a new methodology to improve the conditions of shrimp farming. In this study, a cDNA library was carried out with the purpose of reviewing the transcripts related to the function of the immune system of shrimp. L. vannamei. In order to prepare transcripts RNAseq methodology Sequency Next Generation (NGS) was followed, and the readings were subjected to analysis TRINITY for preparing a Novo assembling. The transcripts that were found in the shotgun bank, 440 were selected from an immune type. In this selection 336 transcripts were obtained and they were associated with the immune system with significant probability values, from this group of genes it was proceeded to perform their classification according to their immune function. It could be obtained transcripts associated with Endocytosis, virus defense function, bacteria defense function, fungi defense function, humoral function, signaling cascades immune response and other immunity functions.
This thesis was conducted with the samples obtained from a maturation laboratory that is located in the coastal area of Mar Bravo in the Province of Santa Elena, the aim of this project was to study the immune status of the shrimp L. vannamei, which an early recognition and use of bioinformatics, they are presented as a new methodology to improve the conditions of shrimp farming. In this study, a cDNA library was carried out with the purpose of reviewing the transcripts related to the function of the immune system of shrimp. L. vannamei. In order to prepare transcripts RNAseq methodology Sequency Next Generation (NGS) was followed, and the readings were subjected to analysis TRINITY for preparing a Novo assembling. The transcripts that were found in the shotgun bank, 440 were selected from an immune type. In this selection 336 transcripts were obtained and they were associated with the immune system with significant probability values, from this group of genes it was proceeded to perform their classification according to their immune function. It could be obtained transcripts associated with Endocytosis, virus defense function, bacteria defense function, fungi defense function, humoral function, signaling cascades immune response and other immunity functions.
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TRANSCRITOS, SHOTGUN, L. VANNAMEI, CASCADAS DE SEÑALIZACIÓN, RESPUESTA INMUNE