Estudio de marcadores placentarios en circulación fetal de gestantes complicadas con preeclampsia severa, Hospital Gineco-Obstétrico Enrique C. Sotomayor, 2014
No hay miniatura disponible
Fecha
2016
Autores
Título de la revista
ISSN de la revista
Título del volumen
Editor
Universidad de Guayaquil. Facultad de Ciencias Médicas. Escuela de Graduados
Resumen
Antecedentes: La preeclampsia (PE) es un desorden mutisistémico y multifactorial. Se cree que en esta patología existe un desbalance entre factores angiogénicos y antiangiogénicos durante el inicio de la placentación. Varios marcadores bioquímicos han sido estudiados para la predicción de la enfermedad como el factor de crecimiento placentario (PlGF), la beta gonadotropina coriónica humana libre (ß-hCG) y la proteína A plasmática asociada al embarazo (PAPP-A) en sangre materna pero nunca en circulacion fetal. Objetivo: Medir las concentraciones de PIGF, ß-hCG libre y PAPP-A en circulación fetal (arteria y vena umbilical) en gestantes complicadas con Preeclampsia severa cerca de término y en mujeres sanas, pareadas por paridad, edad materna y gestacional. Métodos: Se tomó una muestra de sangre umbilical de vena (n= 20) y arteria (n= 20) a 20 gestantes complicadas con PE severa y mujeres snaas al momento de nacer, para medir PIGF, ß-hCG libre y PAPP-A mediante fluoroinmunoensayo. Resultados: Las gestantes complicadas con Preeclampsia severa mostraron concentraciones significativamente menores de PIGF en circulación arterial y venosa fetal en comparación con las mujeres sanas (25,1 ± 4,1 pg/ml vs 36,9 ± 24,9 ng/ml; P < 0.05 y 23,6 ± 5,5 pg/ml vs 33,9 ± 34,7 ng/ml; P < 0,05) y de ß-hCG libre (33,9 ± 4,3 ng/ml vs 17,2 ± 4,0 ng/ml; P < 0.001 y 30,1 ± 5,24 pg/ml vs 13,7 ± 3,3 ng/ml; P < 0,05). Conclusiones: Al igual que en sangre materna se encontraron niveles menores de PIGF en circulación fetal en gestaciones cerca de término complicadas con PE severa; junto a niveles mayores de PAPP-A y ß-hCG libre.
Background: Preeclampsia is a multifactorial and multisystemic disorder. Several biochemical markers have been studied for the prediction of disease such as placental growth factor (PIGF), the free beta human chorionic gonadotropin (ß-hCG) and plasma protein associated with pregnancy (PAPP-A). These have been studied in maternal blood but not in the fetal circulation. Objective: To measure the concentration of PIGF, ß-hCG and PAPP-A in fetal circulation (umbilical artery and vein) in near term pregnancies complicated with severe PE; as well as in their controls matched for parity, maternal and gestational age. Method: A blood sample was taken from the umbilical vein (n= 20) and artery (n= 20) of 20 pregnant women with severe PE in order to measure the proposed analyses using fluoroimmunoassay. Results: Pregnancies complicated with severe PE showed significantly lower PIGF levels in fetal circulation as compared to controls (25,1 ± 4,1 pg/ml vs 36,9 ± 24,9 ng/ml; P < 0.05 y 23,6 ± 5,5 pg/ml vs 33,9 ± 34,7 ng/ml; P < 0,05) and free ß-hCG (33,9 ± 4,3 ng/ml vs 17,2 ± 4,0 ng/ml; P < 0.001 y 30,1 ± 5,24 pg/ml vs 13,7 ± 3,3 ng/ml; P < 0,05). Conclusions: As in maternal blood, lower PIGF levels were found in fetal circulation in near term pregnancies complicated with severe PE; together with higher PAPP-A and free ß-hCG levels.
Background: Preeclampsia is a multifactorial and multisystemic disorder. Several biochemical markers have been studied for the prediction of disease such as placental growth factor (PIGF), the free beta human chorionic gonadotropin (ß-hCG) and plasma protein associated with pregnancy (PAPP-A). These have been studied in maternal blood but not in the fetal circulation. Objective: To measure the concentration of PIGF, ß-hCG and PAPP-A in fetal circulation (umbilical artery and vein) in near term pregnancies complicated with severe PE; as well as in their controls matched for parity, maternal and gestational age. Method: A blood sample was taken from the umbilical vein (n= 20) and artery (n= 20) of 20 pregnant women with severe PE in order to measure the proposed analyses using fluoroimmunoassay. Results: Pregnancies complicated with severe PE showed significantly lower PIGF levels in fetal circulation as compared to controls (25,1 ± 4,1 pg/ml vs 36,9 ± 24,9 ng/ml; P < 0.05 y 23,6 ± 5,5 pg/ml vs 33,9 ± 34,7 ng/ml; P < 0,05) and free ß-hCG (33,9 ± 4,3 ng/ml vs 17,2 ± 4,0 ng/ml; P < 0.001 y 30,1 ± 5,24 pg/ml vs 13,7 ± 3,3 ng/ml; P < 0,05). Conclusions: As in maternal blood, lower PIGF levels were found in fetal circulation in near term pregnancies complicated with severe PE; together with higher PAPP-A and free ß-hCG levels.
Descripción
PDF
Palabras clave
Embarazo, Preeclampsia, Marcadores bioquímicos, Hospital Gineco-Obstétrico Enrique C. Sotomayor, Cantón Guayaquil, Ecuador, Circulación placentaria, Mujeres embarazadas