Marcadores bioquímicos involucrados en la predicción de preeclampsia y su relación con el polimorfismo rs4680 del gen catecol-o-metiltransferasa en circulación fetal.
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2019
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Universidad de Guayaquil. Facultad de Ciencias Químicas
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Antecedentes: Investigaciones han determinado que hay varios parámetros bioquímicos relacionados con la preeclampsia que permitirían encontrar antecedentes para la sospecha del inicio de la enfermedad. Pese a esto no se rechaza la idea de que el genoma juega un papel importante en el desarrollo de la patología. Objetivo: Establecer la relación entre los marcadores bioquímicos involucrados en la predicción de la preeclampsia severa con el polimorfismo rs4680 del gen catecol-O-Metil transferasa en circulación fetal. Metodología: este fue un estudio de casos y controles. Para el efecto se utilizaron muestras sanguíneas de cordón umbilical (arteria y vena) criopreservadas de mujeres complicadas con PE severa (40 casos: arteria, n=20 y vena=20) y sus respectivos
40 controles (arteria, n=20 y vena, n=20). Se extrajo ADN y luego se amplificó el polimorfismo rs4680. Además, se midieron los niveles de varios marcadores bioquímicos vinculados a la preeclampsia en arteria y vena umbilical. El análisis se realizó utilizando el paquete estadístico epiinfo™ 7. Resultados: se encontraron niveles menores de factor de crecimiento placentario y mayores de proteína A asociada al embarazo y beta gonadotropina coriónica humana libre (en vena y arteria umbilical) en casos en comparación con los controles. Hubo una tendencia no significativa de niveles menores de factor de crecimiento placentario (pg/ml) en vena umbilical y mayores de proteína A asociada al embarazo y gonadotropina coriónica humana libre según las variantes génicas del polimorfismo rs4680. Conclusión: los niveles de marcadores bioquímicos mostraron diferencias en relación con las variantes génicas del polimorfismo rs4680, aunque estas fueron no significativas posiblemente debido al tamaño muestral. Se requiere de más investigación en este particular.
Background: Investigations have determined that there are several biochemical parameters related to preeclampsia (PE) that would allow raising antecedents for the suspicion of the onset of the disease. Despite this, the idea that the genome plays an important role on the rise or decrease of these biochemical indicators is not rejected. Objective: To establish the relationship between the biochemical markers involved in the prediction of severe preeclampsia with the rs4680 polymorphism of the catechol-O-methyl transferase (COMT) gene in the fetal circulation. Methodology: this was a case-control study. For this purpose, blood samples of cryopreserved umbilical cord (artery and vein) of women complicated with severe PE were used (40 cases: artery, n = 20 and vein = 20) and their respective 40 controls (artery, n = 20 and vein, n = 20). DNA was extracted and then the rs4680 polymorphism was amplified. In addition, the levels of several biochemical markers linked to PE in the umbilical artery and vein were measured. The analysis was carried out using the epiinfo ™ 7 statistical package. Results: Lower levels of Placental Growth Factor and higher levels of protein A associated with pregnancy and free β-human chorionic gonadotropin (in vein and umbilical artery) were found in cases compared to controls. There was a non-significant trend in the umbilical vein of lower levels of Placental Growth Factor (pg / mL) and higher protein A associated with pregnancy and free β-human chorionic gonadotropin levels according to the genetic variants of the rs4680 polymorphism. Conclusion: The levels of biochemical markers showed differences in relation to the gene variants of the rs4680 polymorphism, although these were not significant, possibly due to the sample size. More research is required in this regard.
Background: Investigations have determined that there are several biochemical parameters related to preeclampsia (PE) that would allow raising antecedents for the suspicion of the onset of the disease. Despite this, the idea that the genome plays an important role on the rise or decrease of these biochemical indicators is not rejected. Objective: To establish the relationship between the biochemical markers involved in the prediction of severe preeclampsia with the rs4680 polymorphism of the catechol-O-methyl transferase (COMT) gene in the fetal circulation. Methodology: this was a case-control study. For this purpose, blood samples of cryopreserved umbilical cord (artery and vein) of women complicated with severe PE were used (40 cases: artery, n = 20 and vein = 20) and their respective 40 controls (artery, n = 20 and vein, n = 20). DNA was extracted and then the rs4680 polymorphism was amplified. In addition, the levels of several biochemical markers linked to PE in the umbilical artery and vein were measured. The analysis was carried out using the epiinfo ™ 7 statistical package. Results: Lower levels of Placental Growth Factor and higher levels of protein A associated with pregnancy and free β-human chorionic gonadotropin (in vein and umbilical artery) were found in cases compared to controls. There was a non-significant trend in the umbilical vein of lower levels of Placental Growth Factor (pg / mL) and higher protein A associated with pregnancy and free β-human chorionic gonadotropin levels according to the genetic variants of the rs4680 polymorphism. Conclusion: The levels of biochemical markers showed differences in relation to the gene variants of the rs4680 polymorphism, although these were not significant, possibly due to the sample size. More research is required in this regard.
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MARCADORES BIOQUÍMICOS, PREECLAMPSIA, HOMOCISTEÍNA, POLIMORFISMO, RS4680