Evaluación de diferentes dosis de la citocinina BAP en la propagación in vitro de vainilla tahitensis
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2020-10
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Facultad de Ciencias Agrarias Universidad de Guayaquil
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La presente investigación se realizó en el Laboratorio de Biotecnología AGROVITROPARIS, ubicado en la ciudad de Guayaquil-parroquia Pascuales avda. Narcisa de Jesús (Metrópolis II etapa H), comenzando el 4 de junio del 2020 hasta el 12 de septiembre de 2020. Se estudiaron seis dosis de BAP (0,50 ml/L, 1,00 ml/L, 1,50 ml/L, 1,75 ml/L, 2,00 ml/L y 2,50 ml/L) más un tratamiento testigo con cinco repeticiones, se usó el diseño completamente al azar, de acuerdo con los resultados se concluyó lo siguientes: 1) La dosis de 2,0 ml de BAP permitió la mejor micropropagación del cultivar de vainilla con 5 y 6 brotes a los 60 y 90 días después de la siembra; 2) La mayor longitud de la raíz se presentó con la dosis de 1,75 ml con 7 cm de longitud; 3) En la variable altura de planta y número de hoja, la dosis de 2,0 ml presentó la mayor altura de planta con 18 cm y 6 cm a los 90 y 60 días de la siembra y el mayor número de hoja
con 7 y 5 hojas a los 90 y 60 días de la siembra y 4) En el análisis económico de
los tratamientos del ensayo, el mayor beneficio neto se produjo con el tratamiento 2 (BAP en dosis de 1,0 ml) con U.S.D. 116, 00,
The following research was conducted at the AGROVITROPARIS Biotechnology Laboratory property of Mrs. Laura Paris Moreno, University Professor of the Agriculture Sciences Faculty, located in the city of Guayaquil-Pascuales, parish “Narcisa de Jesús” (Metrópolis II stage H). The present project ran from June 4th to September 12th, 2020. Six doses of BAP (0,50 ml / L, 1,00 ml / L, 1,50 ml / L, 1,75 ml / L, 2,00 ml / L and 2,50 ml / L) plus a control treatment with five repetitions, the fully randomized design was used, according to the outcomes, it was concluded that: 1) The 2.0 ml dose of BAP achieved the best micropropagation of the vanilla cultivar with 5 and 6 shoots at 60 and 90 days after sowing; 2) The greatest length of the root occurred with the dose of 1,75 ml with 7 cm in length; 3) In the variable plant height and leaf number, the 2,0 ml dose presented the greatest plant height with 18 cm and 6 cm at 90 and 60 days after sowing and the largest leaf number with 7 and 5 leaves at 90 and 60 days after sowing. 4) As for the economic analysis of the trial, the control treatment presented the lowest cost with USD 14,00, followed by the dose of 0,5 ml of BAP with USD 24,00, the 2,5 ml dose of BAP obtained the highest expenditure with USD 64,00.
The following research was conducted at the AGROVITROPARIS Biotechnology Laboratory property of Mrs. Laura Paris Moreno, University Professor of the Agriculture Sciences Faculty, located in the city of Guayaquil-Pascuales, parish “Narcisa de Jesús” (Metrópolis II stage H). The present project ran from June 4th to September 12th, 2020. Six doses of BAP (0,50 ml / L, 1,00 ml / L, 1,50 ml / L, 1,75 ml / L, 2,00 ml / L and 2,50 ml / L) plus a control treatment with five repetitions, the fully randomized design was used, according to the outcomes, it was concluded that: 1) The 2.0 ml dose of BAP achieved the best micropropagation of the vanilla cultivar with 5 and 6 shoots at 60 and 90 days after sowing; 2) The greatest length of the root occurred with the dose of 1,75 ml with 7 cm in length; 3) In the variable plant height and leaf number, the 2,0 ml dose presented the greatest plant height with 18 cm and 6 cm at 90 and 60 days after sowing and the largest leaf number with 7 and 5 leaves at 90 and 60 days after sowing. 4) As for the economic analysis of the trial, the control treatment presented the lowest cost with USD 14,00, followed by the dose of 0,5 ml of BAP with USD 24,00, the 2,5 ml dose of BAP obtained the highest expenditure with USD 64,00.
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CULTIVO IN VITRO, VAINILLA, BAP, MEDIO DE CULTIVO