Estudio etnobotánico, químico y antioxidante de Justicia secunda Vahl
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2021
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Universidad de Guayaquil. Facultad de Ciencias Químicas
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Los radicales libres son sustancias de origen endógeno y/o exógeno que producen estrés oxidativo y daño celular. Muchas plantas son utilizadas ancestralmente para combatir dolencias atribuidas al estrés oxidativo. La Justicia secunda Vahl, cuyo nombre vulgar es insulina, que por los resultados de la encuesta realizada a la población es utilizada para reducir los niveles de glucosa en sangre, hipertensión y miomas uterinos que son los más conocidos en la sociedad, por lo cual es necesario evaluar la concentración de la Actividad Antioxidante y Polifenoles Totales en el extracto hidroalcohólico. Se realizó un tamizaje fitoquímico pre-eliminar el cual presentó metabolitos secundarios como: alcaloides, quinonas, triterpenos y/o esteroides, saponinas, fenoles/tanino, flavonoides y lactonas/cumarinas. Además, se determinó la concentración de la actividad antioxidante por el método DPPH en los estándares de ácido ascórbico (82,59µg/ml) y en ácido gálico (293,22µg/ml), mientras que en los polifenoles totales por el método Folin-Ciocalteu se obtuvo (0,05%).
Free radicals are substances of endogenous and / or exogenous origin that produce oxidative stress and cell damage. Many plants are used ancestrally to combat ailments attributed to oxidative stress. Justice second Vahl, whose vulgar name is insulin, which, based on the results of the population survey, is used to reduce blood glucose levels, hypertension and uterine fibroids that are the best known in society, for which it is It is necessary to evaluate the concentration of the Antioxidant Activity and Total Polyphenols in the hydroalcoholic extract. A pre-elimination phytochemical screening was carried out which presented secondary metabolites such as: alkaloids, quinones, triterpenes and / or steroids, saponins, phenols / tannin, flavonoids and lactones / coumarins. In addition, the concentration of antioxidant activity was determined by the DPPH method in the standards of ascorbic acid (82.59µg / ml) and in Gallic acid (293.22µg / ml), while in total polyphenols by the Folin- method. Ciocalteu was obtained (0.05%).
Free radicals are substances of endogenous and / or exogenous origin that produce oxidative stress and cell damage. Many plants are used ancestrally to combat ailments attributed to oxidative stress. Justice second Vahl, whose vulgar name is insulin, which, based on the results of the population survey, is used to reduce blood glucose levels, hypertension and uterine fibroids that are the best known in society, for which it is It is necessary to evaluate the concentration of the Antioxidant Activity and Total Polyphenols in the hydroalcoholic extract. A pre-elimination phytochemical screening was carried out which presented secondary metabolites such as: alkaloids, quinones, triterpenes and / or steroids, saponins, phenols / tannin, flavonoids and lactones / coumarins. In addition, the concentration of antioxidant activity was determined by the DPPH method in the standards of ascorbic acid (82.59µg / ml) and in Gallic acid (293.22µg / ml), while in total polyphenols by the Folin- method. Ciocalteu was obtained (0.05%).
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JUSTICIA SECUNDA VAHL, ACTIVIDAD ANTIOXIDANTE, POLIFENOLES TOTALES, TAMIZAJE FITOQUÍMICO PRELIMINAR