Propagación in vitro de la orquidea "Phalaenopsis violacea" a través de la vara floral
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2014
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Universidad de Guayaquil; Facultad de Ciencias Naturales
Resumen
El presente trabajo tiene como objetivo desarrollar la micropropagación de Phalaenopsis violacea mediante las técnicas de cultivo en la vara floral. Para obtener la formación de cuerpos protocórmicos, iniciando con el procedimiento de desinfección con la utilización de hipoclorito de sodio al 4% y tween 20% (2 gotas/100 ml). Se utilizaron 4 tipos de tratamientos: A, B, C, D, de los cuales el D presentó el 10% de contaminación, mientras que en el B el 25%, en el C el 40% y en el A el 50%. Los primeros cuerpos protocórmicos fueron observados a las siete semanas del cultivo, se evaluó el crecimiento y supervivencia. Para el análisis estadístico se realizó un diseño completamente al azar, mediante un análisis de varianza y la prueba de tukey. Los resultados obtenidos en esta investigación confirman que sí es posible la micropropagación de las varas florales en Phalaenopsis violacea. En el tratamiento D que es el medio de cultivo Vancin & Went con adición de agua de coco, se obtuvo las respuestas más favorables, con un 90% de supervivencia de acuerdo a las siguientes mediciones: Longitud del brote (2.9 cm), número de hojas (5), longitud de la raíz (1.6 cm). Los resultados muestran que hay diferencias significativas entre los diferentes tratamientos y que estos influyen de manera positiva en el crecimiento de las plántulas, ayudando en el desarrollo de las hojas y raíces.
The aim of the present work is to develop the microspreading of Phalaenopsis violacea by culture techniques from flowers buds. In order to obtaining the formation of protocormic bodies, as beginning of disinfection have been used sodium hypochlorite 4% and Tween 20% (2 drops / 100 ml). Four types of treatments have been used: A, B, C, D, of which D showed 10% of pollution, while 25% in B, 40% in C and 50% in A. The first protocormic bodies have been observed at seventh week of growing, survival and growth have been evaluated. For the statistical analysis was performed a completely randomized design using analysis of variance and tukey's test. The results obtained in this research confirmed that microspreading floral rod is possible Phalaenopsis violacea. In treatment D, that is the culture media of growing of Vancin & Went with addition of coconut water, was obtained the most favorable response with 90% survival, according to the following measurements: length of shoot (2.9 cm), number of leaves (5), root length (1.6 cm). The results have demonstrated that there are significant differences among the several treatments and that those perform a positively influences on seedling growth, allowing in the development of leaves and roots.
The aim of the present work is to develop the microspreading of Phalaenopsis violacea by culture techniques from flowers buds. In order to obtaining the formation of protocormic bodies, as beginning of disinfection have been used sodium hypochlorite 4% and Tween 20% (2 drops / 100 ml). Four types of treatments have been used: A, B, C, D, of which D showed 10% of pollution, while 25% in B, 40% in C and 50% in A. The first protocormic bodies have been observed at seventh week of growing, survival and growth have been evaluated. For the statistical analysis was performed a completely randomized design using analysis of variance and tukey's test. The results obtained in this research confirmed that microspreading floral rod is possible Phalaenopsis violacea. In treatment D, that is the culture media of growing of Vancin & Went with addition of coconut water, was obtained the most favorable response with 90% survival, according to the following measurements: length of shoot (2.9 cm), number of leaves (5), root length (1.6 cm). The results have demonstrated that there are significant differences among the several treatments and that those perform a positively influences on seedling growth, allowing in the development of leaves and roots.
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ÁCIDO NAFTALÉNACÉTICO (ANA), BENCIL AMINOPURINA (BAP), IN VITRO, ORQUÍDEA, PHALAENOPSIS VIOLACEA
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APA