Bioensayos de Plomo en renacuajos de Rhinella horribilis: ¿Cómo afecta su desarrollo y supervivencia?
Fecha
2023
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Facultad de Ciencias Naturales. Universidad de Guayaquil
Resumen
El plomo es un metal pesado que tiene efectos perjudiciales en los ecosistemas
acuáticos, y su inducción al medio ambiente por el ser humano plantea
preocupaciones ambientales. En este estudio investigamos el impacto de los
niveles de plomo con base en el límite máximo permisible definido en el Acuerdo
Ministerial 097-A sobre recursos hídricos, en el desarrollo larvario del sapo de
caña mesoamericano, Rhinella horribilis. Los renacuajos utilizados en el
experimento provenían de la misma fuente y se encontraban en estado larvario
17 de Gosner. Se prepararon ocho niveles de tratamiento de soluciones de
cloruro de plomo (que van desde 0,001 a 5,000 mg/L) con base en las normas
vigentes de Ecuador. El experimento se llevó a cabo bajo condiciones climáticas
tropicales. Evaluamos los efectos de estas concentraciones de plomo en función
de la supervivencia, la morfometría, el comportamiento de natación y la
morfología de las larvas. Los análisis toxicológicos y estadísticos revelaron
efectos significativos para todas las concentraciones de plomo probadas. Se
observa una CL50 de 0.0074 mg/L, una reducción de las de la longitud en las
concentraciones mayores a 0.050 mg/L, y alteración en la conducta natatoria a
partir de 0.001 mg/L. En consecuencia, es necesario llevar a cabo una revisión
exhaustiva de la normativa, para salvaguardar la vida silvestre, específicamente
las especies de agua dulce en base a este estudio.
Lead is a heavy metal that has detrimental effects on aquatic ecosystems, and its human-induced contamination of the environment raises environmental concerns. In this study, we investigated the impact of lead levels based upon the maximum permissible limit defined in Ministerial Agreement 097-A on water resources, specifically on the larval development of the Mesoamerican Cane Toad Rhinella horribilis. The tadpoles used in the experiment came from the same source and were at Gosner larval stage 17. Eight treatment levels of lead chloride solutions (ranging from 0.001 to 5.000 mg/L) were prepared based on currently regulations of Ecuador. The experiment was carried out under tropical climatic conditions. We assessed the effects of these lead concentrations based on survival, morphometry, swimming behavior, and morphology of the larvae. Toxicological and statistical analyses unveiled significant effects for all lead concentrations tested. The LC50 value is 0.0074 mg/L, the reduction in length was concentrations greater than 0.050 mg/l, and altered swimming at 0.001 mg/L and above. Consequently, it is necesaary to carry out an exhaustive review of the regulations, in order to safeguard wildlife specifically freshwater species base don this study.
Lead is a heavy metal that has detrimental effects on aquatic ecosystems, and its human-induced contamination of the environment raises environmental concerns. In this study, we investigated the impact of lead levels based upon the maximum permissible limit defined in Ministerial Agreement 097-A on water resources, specifically on the larval development of the Mesoamerican Cane Toad Rhinella horribilis. The tadpoles used in the experiment came from the same source and were at Gosner larval stage 17. Eight treatment levels of lead chloride solutions (ranging from 0.001 to 5.000 mg/L) were prepared based on currently regulations of Ecuador. The experiment was carried out under tropical climatic conditions. We assessed the effects of these lead concentrations based on survival, morphometry, swimming behavior, and morphology of the larvae. Toxicological and statistical analyses unveiled significant effects for all lead concentrations tested. The LC50 value is 0.0074 mg/L, the reduction in length was concentrations greater than 0.050 mg/l, and altered swimming at 0.001 mg/L and above. Consequently, it is necesaary to carry out an exhaustive review of the regulations, in order to safeguard wildlife specifically freshwater species base don this study.
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TOXICIDAD POR PLOMO, TOXICIDAD CONDUCTUAL, TOXICOLOGIA ACUATICA, TOXICOLOGIA DE ANFIBIOS