Utilidad de pcr multiplex ivd para el diagnóstico complementario de patógenos gastrointestinales
Fecha
2022
Autores
Título de la revista
ISSN de la revista
Título del volumen
Editor
Universidad de Guayaquil. Facultad de Ciencias Médicas. Escuela de Graduados
Resumen
Antecedentes: La gastroenteritis infecciosa es una enfermedad con alta morbilidad y causa de muerte, en los pacientes oncológicos tratados con quimioterapia, esto suele manifestarse por la presencia de neutropenia. Objetivo: determinar la utilidad de la prueba PCR multiplex IVD para el diagnóstico complementario de patógenos gastrointestinales en pacientes con gastroenteritis ingresados en el Hospital SOLCA de Guayaquil del 1 de febrero del 2020 al 31 de agosto 2021. Métodos: la siguiente investigación fue de tipo cuantitativo, con diseño descriptivo, correlacional, no experimental, retrospectivo y transversal; donde se recolecto información por medio de las historias clínicas de los pacientes oncológicos con diagnóstico de gastroenteritis, que se les haya realizado coprocultivo y prueba PCR multiplex IVD en el Hospital SOLCA del 1 de febrero del 2020 al 31 de agosto del 2021. Resultados: Características de los pacientes: Edad 6 a 12 años 20,4% y 1 a 5 años 17,6%. Sexo: masculino 62,0%. Síntomas: diarrea 100%, astenia 47,7%, fiebre 36,7% y dolor abdominal 28,4%. Agentes patógenos por PCR Multiplex: EPEC 8,3%, EAEC 3,7%, Clostridium difficile 3,7%. Agentes patógenos por cultivo: Escherichia Coli 13,8%, Klebsiella Neumoniae Species 8,3%, Klebsiella Neumoniae 7,3%. PCR Multiplex 53,7% detectado y 44,0% no detectado. Cultivo: 43,1% detectado y 56,0% no detectado. Conclusiones: Los resultados evidencian la eficiencia del PCR sobre el Coprocultivo para el diagnóstico oportuno de agentes patógenos asociados a enfermedades diarreicas infecciosas.
Palabras clave: Gastroenteritis, Sensibilidad y Especificidad, Diarrea, Reacción multiplex en cadena de la polimerasa
Background: Infectious gastroenteritis is a disease with high morbidity and cause of death in poor countries of the world. In cancer patients treated with chemotherapy, this is usually manifested by the presence of neutropenia. Objective: determine the usefulness of the multiplex IVD PCR test for the complementary diagnosis of gastrointestinal pathogens in patients with gastroenteritis admitted to the SOLCA Hospital in Guayaquil from February 1, 2020 to August 31. of 2021. Methods: the following research was quantitative, with a descriptive, correlational, non-experimental, retrospective and cross- sectional design; where information was collected through the medical records of oncology patients diagnosed with gastroenteritis, who underwent coproculture and multiplex IVD PCR test at SOLCA Hospital from February 1, 2020 to August 31, 2021. Results: Characteristics of the patients: Age 6 to 12 years 20.4% and 1 to 5 years 17.6%. Male gender 62.0%. Symptoms: diarrhea 100%, asthenia 47.7%, fever 36.7% and abdominal pain 28.4%. Pathogens by Multiplex PCR: EPEC 8.3%, EAEC 3.7%, Clostridium difficile 3.7%. Pathogens per culture: Escherichia Coli 13.8%, Klebsiella Pneumoniae Species 8.3%, Klebsiella Pneumoniae 7.3%. Multiplex PCR 53.7% detected and 44.0% not detected. Culture: 43.1% detected and 56.0% undetected. Conclusions: The results show the efficiency of PCR over coproculture for the timely diagnosis of pathogens associated with infectious diarrheal diseases. Keywords: Gastroenteritis, Sensitivity and Specificity, Diarrhea, Multiple polymerase chain reaction
Background: Infectious gastroenteritis is a disease with high morbidity and cause of death in poor countries of the world. In cancer patients treated with chemotherapy, this is usually manifested by the presence of neutropenia. Objective: determine the usefulness of the multiplex IVD PCR test for the complementary diagnosis of gastrointestinal pathogens in patients with gastroenteritis admitted to the SOLCA Hospital in Guayaquil from February 1, 2020 to August 31. of 2021. Methods: the following research was quantitative, with a descriptive, correlational, non-experimental, retrospective and cross- sectional design; where information was collected through the medical records of oncology patients diagnosed with gastroenteritis, who underwent coproculture and multiplex IVD PCR test at SOLCA Hospital from February 1, 2020 to August 31, 2021. Results: Characteristics of the patients: Age 6 to 12 years 20.4% and 1 to 5 years 17.6%. Male gender 62.0%. Symptoms: diarrhea 100%, asthenia 47.7%, fever 36.7% and abdominal pain 28.4%. Pathogens by Multiplex PCR: EPEC 8.3%, EAEC 3.7%, Clostridium difficile 3.7%. Pathogens per culture: Escherichia Coli 13.8%, Klebsiella Pneumoniae Species 8.3%, Klebsiella Pneumoniae 7.3%. Multiplex PCR 53.7% detected and 44.0% not detected. Culture: 43.1% detected and 56.0% undetected. Conclusions: The results show the efficiency of PCR over coproculture for the timely diagnosis of pathogens associated with infectious diarrheal diseases. Keywords: Gastroenteritis, Sensitivity and Specificity, Diarrhea, Multiple polymerase chain reaction
Descripción
PDF
Palabras clave
GASTROENTERITIS, SENSIBILIDAD Y ESPECIFICIDAD, DIARREA, REACCION EN CADENA DE LA POLIMERASA MULTIPLEX, EPIDEMIOLOGIA DESCRIPTIVA, ESTUDIOS RETROSPECTIVOS, HOSPITAL SOLCA DE GUAYAQUIL, CANTON GUAYAQUIL, ECUADOR