Determinación de niveles de anticuerpos antirrábicos de perros ingresados en el laboratorio de diagnóstico de rabia, salud animal mediante la técnica de sebo neutralización en ratones.
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2013
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Universidad de Guayaquil Facultad de Medicina Veterinaria
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La presente investigación tuvo como objeto obtener más conocimiento acerca del virus de la rabia, para poder determinar los factores de riesgo de esta en nuestro país y con esto mejorar la calidad de vida tanto del can como del hombre. Para llegar a los objetivos citados de esta técnica se tuvo que realizar algunas actividades como la extracción de sangre de la vena radial del antebrazo de los canes ingresados en el Laboratorio de Diagnóstico de rabia, para ser centrifugadas las 50 muestras y guardadas a temperatura -20 ° C hasta su uso. Se realizó varias diluciones para la Dosis letal e inoculación de ratones de 11 a 14 gramos de peso y se observó durante 14 días. Una vez determinada la dosis letal se inactivo los sueros y se procedió hacer diluciones con suero equino, solución de buffer y CVS. Teniendo todas las diluciones hechas se procedió a colocarlos en una estufa por 90 minutos, luego dejándolas por 10 minutos con hielo para proceder a inocular por la vía intracraneal a cada ratón y fueron observados por 14 días anotando diariamente en los protocolos. Se empleo la técnica de Reed & muench obteniendo como resultados la evidencia de anticuerpos en todas las inoculaciones, favoreciendo esta investigación a las campañas realizadas anualmente.
The present investigation was to obtain more knowledge about the virus of rabies, in order to be able to determine the risk factors for this in our country and with this improve the quality of life both of the can as the man. To reach the stated objectives of this technique had to perform some activities such as drawing blood from the radial forearm vein of dogs admitted to the Laboratory Diagnosis of rabies, for the 50 samples centrifuged and stored at temperature -20 ° C until use. We performed several dilutions and lethal dose for mice inoculated 11 to 14 grams of weight and observed for 14 days. Having found the lethal dose was inactivated sera and proceeded to horse serum dilutions, buffer solution and CVS. Taking all dilutions made proceeded to place them in an oven for 90 minutes, then leaving for 10 minutes with ice to proceed to inoculate each mouse intracranial and were observed daily for 14 days by scoring the protocols.They use the technique of Reed & Muench obtaining as results the presence of antibodies in all inoculations, favoring this research to campaigns annually.
The present investigation was to obtain more knowledge about the virus of rabies, in order to be able to determine the risk factors for this in our country and with this improve the quality of life both of the can as the man. To reach the stated objectives of this technique had to perform some activities such as drawing blood from the radial forearm vein of dogs admitted to the Laboratory Diagnosis of rabies, for the 50 samples centrifuged and stored at temperature -20 ° C until use. We performed several dilutions and lethal dose for mice inoculated 11 to 14 grams of weight and observed for 14 days. Having found the lethal dose was inactivated sera and proceeded to horse serum dilutions, buffer solution and CVS. Taking all dilutions made proceeded to place them in an oven for 90 minutes, then leaving for 10 minutes with ice to proceed to inoculate each mouse intracranial and were observed daily for 14 days by scoring the protocols.They use the technique of Reed & Muench obtaining as results the presence of antibodies in all inoculations, favoring this research to campaigns annually.
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PATOLOGIA, EPYZOOTIAS, EPIDEMIOLOGÍA, CANINOS