Caracterización molecular de plantas de plátano Musa spp. modificadas genéticamente para Biofortificación de folato.
Fecha
2019
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Facultad de Ciencias Naturales. Universidad de Guayaquil
Resumen
Los cultivos de plátano y banano (Musa spp.) se enmarcan como los rubros socioeconómicos más altos en Ecuador. La implementación de mejoras génicas vegetales, a partir del uso de herramientas moleculares como la transformación mediada por Agrobacterium tumefaciens, permiten potencializar las características nativas de la especie como en este caso la integración de genes relacionados en la síntesis de folato (vitamina B9). En este trabajo se evalúan las características morfológicas (altura de la planta y grosor del pseudotallo) de plantas las transformadas con tres constructors denominados como, 1 pMOD35hMaG con el gen GTPCHI; constructo 4 pMOD35hMaGA con el gen ADCS, y el constructo 5 pMOD35hAtGA con los genes GTPCHI-ADCS. Además, se evaluó la estabilidad de la integración del ADN recombinante en el genoma de las plantas. Las variables morfológicas mediante el análisis estadístico no paramétrico de Kruskal–Wallis (p < 0.05) demostraron que no hay diferencias (p = 0.12) en la altura entre todos los tratamientos, mientras que en el grosor del pseudotallo hubo diferencias entre el constructo 1 y 2 (p = 0.01). Por otro lado, de las 47 accesiones analizadas por PCR, se encontró que todas fueron estables, dando como inserciones positivas. Estos resultados determinan, que las características morfológicas no se ven afectadas por las modificaciones genéticas y que la transformación por A. tumefaciens presenta una alta estabilidad de su ADN-T.
Plantain and banana crops (Musa spp.) they are framed as the highest socioeconomic items in Ecuador, the implementation of plant gene enhancements, from the use of molecular tools such as Agrobacterium tumefaciens mediated transformation potentialize the native characteristics of the species as in this case the integration of related genes into folate synthesis (vitamin B9), this work evaluates the morphological characteristics (plant height and pseudostem thickness) of the transformed plants with three constructors named as, 1 pMOD35hMaG with the GTPCHI gene; construct 4 pMOD35hMaGA with ADCS gene, and the construct 5 pMOD35hAtGA with the GTPCHI-ADCS genes. Also the stability of the integration of recombinant DNA into the plant genome was assessed. Morphological variables using the nonparametric statistical analysis of Kruskal–Wallis (p < 0.05) proved that there are no differences (p = 0.12) in the height between all treatments, while in the thickness of the pseudostem there were differences between construct 1 and 2 (p = 0.01). On the other hand, of the 47 accessions analyzed by PCR, it was found that all were stable, giving as positive insertions. These results determine that morphological characteristics are not affected by genetic modifications and that the transformation by A. tumefaciens a high stability of its T-DNA.
Plantain and banana crops (Musa spp.) they are framed as the highest socioeconomic items in Ecuador, the implementation of plant gene enhancements, from the use of molecular tools such as Agrobacterium tumefaciens mediated transformation potentialize the native characteristics of the species as in this case the integration of related genes into folate synthesis (vitamin B9), this work evaluates the morphological characteristics (plant height and pseudostem thickness) of the transformed plants with three constructors named as, 1 pMOD35hMaG with the GTPCHI gene; construct 4 pMOD35hMaGA with ADCS gene, and the construct 5 pMOD35hAtGA with the GTPCHI-ADCS genes. Also the stability of the integration of recombinant DNA into the plant genome was assessed. Morphological variables using the nonparametric statistical analysis of Kruskal–Wallis (p < 0.05) proved that there are no differences (p = 0.12) in the height between all treatments, while in the thickness of the pseudostem there were differences between construct 1 and 2 (p = 0.01). On the other hand, of the 47 accessions analyzed by PCR, it was found that all were stable, giving as positive insertions. These results determine that morphological characteristics are not affected by genetic modifications and that the transformation by A. tumefaciens a high stability of its T-DNA.
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A. TUMEFACIENS, MALNUTRICIÓN, PCR, VITAMINA B9