Aislamiento e Identificación de Cepas Bacterianas de Ambientes Nativos y su Aplicación Biorremediadora en el Cultivo del Camarón Blanco Litopenaeus Vannamei
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2012
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Universidad de Guayaquil. Facultad de Ciencias Naturales
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En el presente trabajo tuvo como objetivo aislar e identificar bacterias con potencial biorremediadora a partir del suelo de dos piscinas de la Camaronera A en la Provincia del El Oro. Catorce morfotipos de bacterias fueron identificadas por técnica bioquímica. Estas bacterias fueron identificadas como Bacillus, Lactobacillus y Pseudomonas y un morfotipo de levadura, posteriormente estas cepas fueron sometidas a pruebas de antagonismo in vitro con el Vibrio sp. demostrando acción significativa de antagonismo, ocasionando la muerte del patógeno. La capacidad biorremediadora de los siete morfotipos en el cultivo de camarón en las piscinas pruebas fue evaluada con la aplicación de dos probióticos elaborados con las bacterias BSM1 (Bacillus sp), BSM2 Bacillus sp), BSM3 (Pseudomonas sp), BSM7 (Lactobacillus plantarum), BMS8 (Pseudomonas sp), BMS10 (Lactobacillus plantarum), BMS11 (Bacillus sp) y la levadura (Sacharomyces serevisae). Los resultados obtenidos mostraron que la biorremediación del suelo, agua y camarón de las piscinas pruebas de las Camaroneras Uno y Dos en la primera producción fue igual que las piscinas control, en la segunda y tercera producción fueron superiores. La presencia de bacterias patógenas, no se evidenció en ninguno de los controles realizados en las piscinas en estudio. La aplicación de los probióticos nativos influyó positivamente sobre el incremento de supervivencia, ganancia de peso, conversión alimentaria y libras de camarón por hectárea a cosecha, que mostró diferencias estadísticas significativas para los días de evaluación que duró la investigación
In the present study aimed to isolate and identify potential bacteria from soil biorremediadora two pools Shrimp A in the province of El Oro Fourteen morphotypes of bacteria were identified by biochemical technique. These bacteria were identified as Bacillus, Pseudomonas and Lactobacillus and yeast morfotipo subsequently these strains were tested for in vitro antagonism with Vibrio sp. demonstrating significant antagonism action, killing the pathogen. The capacity of the seven morphotypes biorremediadora in shrimp farming in the pools was assessed with tests the application of two probiotic bacteria made with BSM1 (Bacillus sp), Bacillus sp BSM2) BSM3 (Pseudomonas sp), BSM7 (Lactobacillus plantarum) , BMS8 (Pseudomonas sp), BMS10 (Lactobacillus plantarum), BMS11 (Bacillus sp) and yeast (Saccharomyces serevisae). The results showed that the bioremediation of soil, water and pools shrimp Shrimp testing of One and Two in the first production was like pools control in the second and third production were higher. The presence of pathogenic bacteria, there was no evidence in any of the controls in the study pools. The application of probiotics positively influenced natives increased survival, weight gain, feed conversion and pounds of shrimp per hectare at harvest, which showed statistically significant differences for day evaluation period of the investigation
In the present study aimed to isolate and identify potential bacteria from soil biorremediadora two pools Shrimp A in the province of El Oro Fourteen morphotypes of bacteria were identified by biochemical technique. These bacteria were identified as Bacillus, Pseudomonas and Lactobacillus and yeast morfotipo subsequently these strains were tested for in vitro antagonism with Vibrio sp. demonstrating significant antagonism action, killing the pathogen. The capacity of the seven morphotypes biorremediadora in shrimp farming in the pools was assessed with tests the application of two probiotic bacteria made with BSM1 (Bacillus sp), Bacillus sp BSM2) BSM3 (Pseudomonas sp), BSM7 (Lactobacillus plantarum) , BMS8 (Pseudomonas sp), BMS10 (Lactobacillus plantarum), BMS11 (Bacillus sp) and yeast (Saccharomyces serevisae). The results showed that the bioremediation of soil, water and pools shrimp Shrimp testing of One and Two in the first production was like pools control in the second and third production were higher. The presence of pathogenic bacteria, there was no evidence in any of the controls in the study pools. The application of probiotics positively influenced natives increased survival, weight gain, feed conversion and pounds of shrimp per hectare at harvest, which showed statistically significant differences for day evaluation period of the investigation
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PROBIÓTICOS, BSM, CAMARÓN