Actividad biológica de Lasiodiplodia theobromae aislado de mango de exportación frente a inductores florales y fungicidas en condiciones de laboratorio
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Fecha
2016
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Facultad de Ciencias Agrarias Universidad de Guayaquil
Resumen
La presente investigación se realizó en los laboratorios de las Facultades de Ciencias Agrarias
y Biotecnología de Ciencias Médicas, de la Universidad de Guayaquil. Los objetivos fueron:
1) Evaluar el efecto de paclobutrazol y nitrato de potasio sobre el crecimiento micelial de
Lasiodiplodia theobromae y 2) Determinar el efecto de siete fungicidas sobre el crecimiento
micelial de Lasiodiplodia theobromae.
Los tratamientos del estudio con inductores florales fueron etrel, nitrato de k y pbz y con
fungicidas imazalil (Fungaflor), azufre (Azufre micronizado), fosetil Al (Aliete), oxido de
cobre (Cuprofit), captan (Captan), sulfato de cobre pentahidratado (Phyton) y thiabendazole
(Mertec).
El hongo fue aislado de ramas de mango cultivar Tommy Atkins en medio de cultivo papa
dextrosa agar (PDA) una vez que fue purificado se multiplicó masivamente para efectuar las
pruebas frente a inductores florales y fungicidas, para ello se colocó en el medio de cultivo
cada uno de los tratamientos y en el centro un disco de 10 mm de crecimiento micelial de L.
theobromae.
Se procedió a medir el crecimiento del hongo, cada 24 horas durante tres días debido al que el
testigo absoluto cubrió toda la caja de 90 mm de diámetro. Los datos se expresaron en mm por
día y porcentaje de crecimiento e inhibición del crecimiento micelial de L. theobromae con
respecto al testigo absoluto.
Los tratamientos con PBZ no permitió el crecimiento de L. theobromae lo que corresponde al
100% de inhibición del crecimiento micelial. Por otra parte, los tratamientos imazalil, oxido
de cobre, Fosetil de aluminio y thiabendazole inhibieron el 100% de crecimiento micelial de
L. theobromae en condiciones de laboratorio.
This research was conducted in the laboratories of the Faculty of Agricultural Sciences and Biotechnology of Medical Sciences, University of Guayaquil. The objectives were to: 1) evaluate the effect of paclobutrazol and potassium nitrate on mycelial growth Lasiodiplodia theobromae and 2) To determine the effect of seven fungicides on mycelial growth Lasiodiplodia theobromae. Study treatments with floral inducers were ethrel nitrate pbz and imazalil fungicide (Fungaflor), sulfur (sulfur micronized), fosetyl Al (Aliete), copper oxide (Cuprofit), captan (Captan), copper sulfate pentahydrate (Python) and thiabendazole (Mertec). The fungus was isolated from branches mango cultivar Tommy Atkins among potato dextrose agar culture medium (PDA) once was purified massively multiplied for testing against floral and fungicidal inducers, for it was placed in culture medium each treatments and center disc 10 mm mycelial growth of L. theobromae. We proceeded to measure the growth of the fungus, every 24 hours for three days due to the absolute control that covered the entire box 90mm in diameter. Data were expressed in mm per day and percentage growth and inhibition of mycelial growth of L. theobromae compared to absolute control. PBZ treatments prevented the growth of L. theobromae corresponding to 100% inhibition of mycelial growth. Moreover, the treatments imazalil, copper oxide, fosetyl aluminum and thiabendazole inhibited 100% mycelial growth of L. theobromae under laboratory conditions.
This research was conducted in the laboratories of the Faculty of Agricultural Sciences and Biotechnology of Medical Sciences, University of Guayaquil. The objectives were to: 1) evaluate the effect of paclobutrazol and potassium nitrate on mycelial growth Lasiodiplodia theobromae and 2) To determine the effect of seven fungicides on mycelial growth Lasiodiplodia theobromae. Study treatments with floral inducers were ethrel nitrate pbz and imazalil fungicide (Fungaflor), sulfur (sulfur micronized), fosetyl Al (Aliete), copper oxide (Cuprofit), captan (Captan), copper sulfate pentahydrate (Python) and thiabendazole (Mertec). The fungus was isolated from branches mango cultivar Tommy Atkins among potato dextrose agar culture medium (PDA) once was purified massively multiplied for testing against floral and fungicidal inducers, for it was placed in culture medium each treatments and center disc 10 mm mycelial growth of L. theobromae. We proceeded to measure the growth of the fungus, every 24 hours for three days due to the absolute control that covered the entire box 90mm in diameter. Data were expressed in mm per day and percentage growth and inhibition of mycelial growth of L. theobromae compared to absolute control. PBZ treatments prevented the growth of L. theobromae corresponding to 100% inhibition of mycelial growth. Moreover, the treatments imazalil, copper oxide, fosetyl aluminum and thiabendazole inhibited 100% mycelial growth of L. theobromae under laboratory conditions.
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FITOPATOLOGIA, CULTIVOS, LASIODIPLODIA, THEOBROMAE