Producción y caracterización de un biopolímero obtenido a partir de residuos agroindustriales del cacao (ccn-51).
No hay miniatura disponible
Fecha
2019-04
Título de la revista
ISSN de la revista
Título del volumen
Editor
Universidad de Guayaquil, Facultad de Ingeniería Química.
Resumen
En el presente trabajo se realizó la producción de un biopolímero empleando los residuos agroindustriales de la industria del cacao CCN-51 y un aislado nativo de Gluconobacter xylinus. Se evaluó los excedentes de cacao como única fuente de carbono en el medio de producción (F1). A su vez se valoró el efecto de dos azúcares en este medio, F2 (sorbitol:excedentes=1:1) y F3 (glicerol:excedentes=1:1), para la producción del
biopolímero. El biopolímero fue caracterizado mediante varios análisis como: microscopía electrónica de barrido (SEM), calorimetría diferencial de barrido (DSC), espectroscopia infrarroja (FTIR), tracción, humedad, color y dimensionamiento de las membranas. Posteriormente se evaluaron las variables con un análisis estadístico ANOVA. Pasados los 15 días de fermentación a una temperatura de 35 oC, se realizó la
caracterización mediante SEM, presentando característica similar a las fibras de celulosa bacteriana; DSC, obteniendo temperaturas de degradación mayor a 190 oC y a 195 oC para la formulación 1 y 2, respectivamente. También se realizó un FTIR obteniendo picos de los grupos funcionales característicos de una celulosa. Se evaluó el rendimiento en base al, medio de producción (RVP) y fuente de carbono (RFC), donde se obtuvieron los resultados 10.43 g/lt y 21.29 g/kg, respectivamente, correspondientes a F2. Mediante análisis estadístico se determinó que las variables evaluadas no presentan diferencias significativas, por lo tanto se aprueba la hipótesis nula.
In the present work, the production of a biopolymer using the agroindustrial residues of the cocoa industry CCN-51 and a native isolate of Gluconobacter xylinus was carried out. The cocoa excess was evaluated as the only carbon source in the production medium (F1). In turn, the effect of two sugars in this medium was evaluated, F2 (sorbitol: cocoa excess = 1:1) and F3 (glycerol: cocoa excess = 1:1), for the production of the biopolymer. The biopolymer was characterized by several analyzes such as: scanning electron microscopy (SEM), differential scanning calorimetry (DSC), Fourier-transform infrared spectroscopy (FTIR), traction, humidity, color and sizing of the membranes. The variables were evaluated with an ANOVA statistical analysis. After 15 days of fermentation at a temperature of 35ºC, the characterization was made by SEM, showing a similar characteristic to bacterial cellulose fibers; DSC, obtaining degradation temperatures higher than 190ºC and at 195ºC for formulation 1 and 2, respectively. An FTIR also obtained peaks of the functional groups characteristic of a cellulose. The yield was evaluated based on the production medium (RVP) and carbon source (RFC), where the results were obtained 10.43 g/L and 21.29 g/kg, respectively, corresponding to F2. Through statistical analysis it was determined that the evaluated variables do not present significant differences, therefore the null hypothesis is approved
In the present work, the production of a biopolymer using the agroindustrial residues of the cocoa industry CCN-51 and a native isolate of Gluconobacter xylinus was carried out. The cocoa excess was evaluated as the only carbon source in the production medium (F1). In turn, the effect of two sugars in this medium was evaluated, F2 (sorbitol: cocoa excess = 1:1) and F3 (glycerol: cocoa excess = 1:1), for the production of the biopolymer. The biopolymer was characterized by several analyzes such as: scanning electron microscopy (SEM), differential scanning calorimetry (DSC), Fourier-transform infrared spectroscopy (FTIR), traction, humidity, color and sizing of the membranes. The variables were evaluated with an ANOVA statistical analysis. After 15 days of fermentation at a temperature of 35ºC, the characterization was made by SEM, showing a similar characteristic to bacterial cellulose fibers; DSC, obtaining degradation temperatures higher than 190ºC and at 195ºC for formulation 1 and 2, respectively. An FTIR also obtained peaks of the functional groups characteristic of a cellulose. The yield was evaluated based on the production medium (RVP) and carbon source (RFC), where the results were obtained 10.43 g/L and 21.29 g/kg, respectively, corresponding to F2. Through statistical analysis it was determined that the evaluated variables do not present significant differences, therefore the null hypothesis is approved
Descripción
Pdf
Palabras clave
Gluconobacter xylinus, Biopolímero, Celulosa Bacteriana, Cacao CCN- 51.