Diagnóstico histopatológico e inmunohistoquimico del linfoma no Hodgkin difuso de céulas grandes B
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2017
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Universidad de Guayaquil. Facultad de Ciencias Médicas. Escuela de Graduados
Resumen
El Linfoma No Hodgkin difuso de células grandes B, es una neoplasia maligna de los
ganglios linfáticos principalmente, estos tumores muestran gran diversidad en cuanto al
comportamiento, morfología, inmunofenotipo y presentación clínica. Representa
actualmente el 65.8% de todos los Linfomas. Ocupa el séptimo lugar de todos los tumores
en el país, siendo más frecuente en el sexo femenino con un 53.7%. El objetivo de esta
investigación será demostrar la sensibilidad y especificidad de la técnica histoquímica e
inmunohistoquímica y la importancia de esta última en el diagnóstico histopatológico de
los Linfomas y como pronóstico en el seguimiento del paciente. Es un estudio cuantitativo,
transversal, analítico, correlacional con análisis bivarial. Se recuperó un total de 41
pacientes. El diagnóstico histopatológico según tinción con Hematoxilina & Eosina más
frecuente fue el Linfoma No Hodgkin difuso de células grandes (35,3%), con una
sensibilidad 42,9%, especificidad 100,0%. La técnica de inmunohistoquímica con un
análisis interobservador resultó con una sensibilidad del 97,06%, especificidad 85,71%, de
todos los marcadores utilizados más frecuentemente positivo en la población de estudio,
fue el CD-20 (35 casos), seguido de LCA (18 casos), CD-79A (12 casos). El diagnóstico
más frecuente según la Inmunohistoquímica fue Linfoma No Hodgkin difuso de células
grandes inmunofenotipo B (65,8%). La técnica de inmunohistoquímica es una prueba
complementaria de gran importancia y validez para el diagnóstico definitivo de Linfoma
no Hodgkin difuso de células grandes B.
Palabras clave: Linfoma no Hodgkin difuso células grandes B, Hematoxilina-Eosina,
Inmunohistoquímica, Algoritmo.
The lymphoma Non-Hodgkin's of cell large B, is a malignant neoplasm of lymph nodes mainly, these tumors have too much diversity about its behavior, morphology, immunophenotype and clinical presentation. At the moment is 65.8% of all lymphomas and has the seventh place of all the tumors in the country, is more frecuently in womens with 53.7%. The proporse on this study will be to realize the sensitivity and specificity of the histochemical and immunohistochemical technical and the importance of inmmunohistochemical in the histopathological diagnosis of lymphomas and it´s prognosis in the following of the patiens. It is a quantitative, transversal, analytical, correlational study with bivariate analysis. A total of 41 patients were recovered. Histopathological diagnosis according to staining with Hematoxylin & Eosin most frequent was diffuse large-cell Non-Hodgkin's Lymphoma (35.3%), with a sensitivity of 42.9%, specificity 100.0%. The immunohistochemical technique with an interobserver analysis resulted in a 97.06% sensitivity, 85.71% specificity, of all the most frequently used positive markers in the study population, it was CD-20 (35 cases), followed by ACL (18 cases), CD-79A (12 cases). The most frequent diagnosis according to Immunohistochemistry was Diffuse Large Cell Non-Hodgkin's Lymphoma immunophenotype B (65.8%). The immunohistochemical technique is a complementary test of great importance and validity for the definitive diagnosis of diffuse large H-cell non-Hodgkin's lymphoma.
The lymphoma Non-Hodgkin's of cell large B, is a malignant neoplasm of lymph nodes mainly, these tumors have too much diversity about its behavior, morphology, immunophenotype and clinical presentation. At the moment is 65.8% of all lymphomas and has the seventh place of all the tumors in the country, is more frecuently in womens with 53.7%. The proporse on this study will be to realize the sensitivity and specificity of the histochemical and immunohistochemical technical and the importance of inmmunohistochemical in the histopathological diagnosis of lymphomas and it´s prognosis in the following of the patiens. It is a quantitative, transversal, analytical, correlational study with bivariate analysis. A total of 41 patients were recovered. Histopathological diagnosis according to staining with Hematoxylin & Eosin most frequent was diffuse large-cell Non-Hodgkin's Lymphoma (35.3%), with a sensitivity of 42.9%, specificity 100.0%. The immunohistochemical technique with an interobserver analysis resulted in a 97.06% sensitivity, 85.71% specificity, of all the most frequently used positive markers in the study population, it was CD-20 (35 cases), followed by ACL (18 cases), CD-79A (12 cases). The most frequent diagnosis according to Immunohistochemistry was Diffuse Large Cell Non-Hodgkin's Lymphoma immunophenotype B (65.8%). The immunohistochemical technique is a complementary test of great importance and validity for the definitive diagnosis of diffuse large H-cell non-Hodgkin's lymphoma.
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Linfoma no Hodgkin, Células grandes B, Hematoxilina, Eosina, Inmunohistoquímica, Algoritmo de diagnóstico, Hospital SOLCA de Guayaquil, Cantón Guayaquil, Ecuador