Determinación de polifenoles totales y actividad antioxidante de la cáscara de caimito morado (Chrysophyllum cainito)
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2023
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Universidad de Guayaquil. Facultad de Ciencias Químicas
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Esta investigación se llevó a cabo mediante análisis experimental acerca del fruto caimito considerado como una fuente importante de nutrientes que brinda gran diversidad en metabolitos secundarios que contribuyen en funciones del organismo, siendo una de ellas la actividad antioxidante, que logran captar radicales libres de oxígeno. Por lo cual se empleó como metodología de análisis preliminar la presencia de metabolitos secundarios por medio del tamizaje fitoquímico, así mismo la cuantificación de polifenoles totales por el método de Follin-Ciocalteu, lo que se alcanzó un valor de 120,26 mg/100g y finalmente en la obtención de cuantificación de la actividad antioxidante se empleó el método DPPH (2,2 difenil-1-picrihidrazilo) dando como resultado 829,07 mg GAE/ ml IC50, dando valores altos para polifenoles totales y valores elevados para la actividad antioxidante con respecto a valores presentes en la cáscara de la misma.
This research was carried out through experimental analysis of the star apple fruit considered as an important source of nutrients that provides great diversity in secondary metabolites that contribute to the functions of the organism, one of which is antioxidant activity, which manages to capture free oxygen radicals. Therefore, the presence of secondary metabolites through phytochemical screening was used as a preliminary analysis methodology, as well as the quantification of total polyphenols by the Follin-Ciocalteu method, which reached a value of 120.26 mg/100g and finally, in obtaining the quantification of the antioxidant activity, the DPPH method (2,2 diphenyl-1-picrihydrazil) was used, resulting in 829.07 mg GAE/ml IC50, giving high values for total polyphenols and high values for activity. antioxidant with respect to values present in the shell of the same.
This research was carried out through experimental analysis of the star apple fruit considered as an important source of nutrients that provides great diversity in secondary metabolites that contribute to the functions of the organism, one of which is antioxidant activity, which manages to capture free oxygen radicals. Therefore, the presence of secondary metabolites through phytochemical screening was used as a preliminary analysis methodology, as well as the quantification of total polyphenols by the Follin-Ciocalteu method, which reached a value of 120.26 mg/100g and finally, in obtaining the quantification of the antioxidant activity, the DPPH method (2,2 diphenyl-1-picrihydrazil) was used, resulting in 829.07 mg GAE/ml IC50, giving high values for total polyphenols and high values for activity. antioxidant with respect to values present in the shell of the same.
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CAIMITO (CHRYSOPHYLLUM CAINITO), ACTIVIDAD ANTIOXIDANTE, POLIFENOLES TOTALES, MÉTODO DPPH