Análisis de fenoles en extracto alcohólico y acuoso del rizoma Phlebodium pseudoaureum (Calaguana) para elaboración de protector solar (CREMA) UV-B
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2023
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Universidad de Guayaquil. Facultad de Ciencias Químicas
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El presente trabajo tuvo como objetivo principal analizar los fenoles en extractos alcohólico y acuoso del rizoma Calaguala para elaboración de protector solar en crema UV-B por medio de la maceración de la muestra con demás ingredientes naturales. En el estudio se realizó para determinar la presencia y concentración de fenoles en los extractos acuosos y alcohólico del Phlebodium pseudoaureum (Calaguala) mediante los métodos de 4-aminoantipirina y el análisis por Folin- Ciolcateu de polifenoles. En un extracto preparado a una concentración 27g/ml, realizando distintas diluciones para efectuar los análisis. Se identificó el fenol que tiene la actividad fotoprotectora el cual es el ácido ferúlico, esté posee una actividad antioxidante y antiinflamatorio. La concentración de fenoles en el extracto alcohólico fue de 65,25mg/ml mientras el extracto acuoso fue de 40,92mg/ml, en una la comparación con la curva de calibración realizada con el estándar de ácido gálico nos indica que la muestra acuosa se encuentra cerca de la absorbancia corregida de 0,098 debido que la absorbancia de la muestra es 0,170 y la muestra alcohólica cerca de la absorbancia de 0,253 donde la absorbancia de la muestra fue 0,304. Los resultados obtenidos nos indican que el extracto adecuado para la elaboración de la crema O/W con actividad de protección solar fue el extracto alcohólico, incluyendo en la elaboración diversos componentes que permitan la formación de la emulsión y excipientes que proporcionen otras propiedades como la hidratación de la piel.
The present work had as main objective to analyze the phenols in alcoholic and aqueous extracts of the Calaguala rhizome for the elaboration of sunscreen in UV- B cream by means of maceration of the sample with other natural ingredients. In the study, it was carried out to determine the presence and concentration of phenols in the aqueous and alcoholic extracts of Phlebodium pseudoaureum (Calaguala) by means of the 4-aminoantipyrine methods and the Folin-Ciolcateu analysis of polyphenols. In an extract prepared at a concentration of 27g/ml, making different dilutions to carry out the analyses. The phenol that has photoprotective activity was identified, which is ferulic acid, it has antioxidant and anti-inflammatory activity. The concentration of phenols in the alcoholic extract was 65.25mg/ml while the aqueous extract was 40.92mg/ml, in a comparison with the calibration curve carried out with the gallic acid standard, it indicates that the aqueous sample was is close to the corrected absorbance of 0.098 because the absorbance of the sample is 0.170 and the alcoholic sample is close to the absorbance of 0.253 where the absorbance of the sample was 0.304. The results obtained indicate that the appropriate extract for the elaboration of the O/W cream with sun protection activity was the alcoholic extract, including in the elaboration various components that allow the formation of the emulsion and excipients that provide other properties such as hydration of the skin.
The present work had as main objective to analyze the phenols in alcoholic and aqueous extracts of the Calaguala rhizome for the elaboration of sunscreen in UV- B cream by means of maceration of the sample with other natural ingredients. In the study, it was carried out to determine the presence and concentration of phenols in the aqueous and alcoholic extracts of Phlebodium pseudoaureum (Calaguala) by means of the 4-aminoantipyrine methods and the Folin-Ciolcateu analysis of polyphenols. In an extract prepared at a concentration of 27g/ml, making different dilutions to carry out the analyses. The phenol that has photoprotective activity was identified, which is ferulic acid, it has antioxidant and anti-inflammatory activity. The concentration of phenols in the alcoholic extract was 65.25mg/ml while the aqueous extract was 40.92mg/ml, in a comparison with the calibration curve carried out with the gallic acid standard, it indicates that the aqueous sample was is close to the corrected absorbance of 0.098 because the absorbance of the sample is 0.170 and the alcoholic sample is close to the absorbance of 0.253 where the absorbance of the sample was 0.304. The results obtained indicate that the appropriate extract for the elaboration of the O/W cream with sun protection activity was the alcoholic extract, including in the elaboration various components that allow the formation of the emulsion and excipients that provide other properties such as hydration of the skin.
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CALAGUALA ( PHLEBODIUM PSEUDOAUREUM), FOTOPROTECTORA, FENOLES